The Key Laboratory of Plant Biotechnology of Liaoning Province, School of Life Science, Liaoning Normal University, Dalian 650081, PR China.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Science, Sun Yat-Sen University, Guangzhou 510275, PR China.
Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005117.
A Gram-stain-positive, non-motile and coccus-shaped bacterium, designated strain LNNU 331112, was isolated from the composite rhizosphere soil of the halophyte (Bunge) Freitag and Schütze, which was collected in Xinjiang, north-west China. Growth occurred at 10-45 °C, pH 6.0-11.0 and in the presence of 0-10 % NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence suggested that strain LNNU 331112 belonged to the genus and showed 95.6, 95.5 and 95.4 % sequence similarities to DSM 45258, CGMCC 4.3532 and CGMCC 1.15478, respectively. The estimated digital DNA-DNA hybridization relatedness values between strain LNNU 331112 and the type strains of DSM 45258, CGMCC 4.3532 and CGMCC 1.15478 were 18.9, 19.3 and 18.3 %, respectively. The average nucleotide identity values between strain LNNU 331112 and DSM 45258, CGMCC 4.3532 and CGMCC 1.15478 were 72.6, 72.7 and 72.3 %, respectively. The genome sequence of strain LNNU 331112 showed 69.0-72.3 % average amino acid identity values in comparison with the related genome sequences of three validly published species. The genome of strain LNNU 331112 was 3.47 Mb, with a DNA G+C content of 68.4 mol%. A total of 3182 genes were identified as protein-coding in strain LNNU 331112. Genomic analysis revealed that a number of genes involved in osmotic pressure regulation, intracellular pH homeostasis and potassium (K) uptake protein were found in strain LNNU 331112. The predominant menaquinones were MK-8 (44.6 %) and MK-7 (55.4 %), which differentiated strain LNNU 331112 from other three recognized species. Major fatty acids (>10 %) were C ω8 (33.8 %), C (23.3 %), C (12.8 %) and summed feature 3 (12.9 %), which also clearly separated strain LNNU 331112 from three recognized species. The polar lipid profile of strain LNNU 331112 included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid, one unidentified phospholipid and two unidentified lipids. According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain LNNU 331112 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LNNU 331112 (=KCTC 39808=CGMCC 1.17107=DSM 103463).
一株革兰氏阳性、非运动和球菌状细菌，被命名为 LNNU 331112 菌株，从中国西北部新疆采集的盐生植物（Bunge）Freitag 和 Schütze 的复合根际土壤中分离得到。该菌在 10-45°C、pH 值 6.0-11.0 和 0-10%NaCl（w/v）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，LNNU 331112 菌株属于 属，与 DSM 45258、CGMCC 4.3532 和 CGMCC 1.15478 分别具有 95.6%、95.5%和 95.4%的 16S rRNA 基因序列相似性。LNNU 331112 菌株与 DSM 45258、CGMCC 4.3532 和 CGMCC 1.15478 的型菌株之间的估计数字 DNA-DNA 杂交相关值分别为 18.9%、19.3%和 18.3%。LNNU 331112 菌株与 DSM 45258、CGMCC 4.3532 和 CGMCC 1.15478 的平均核苷酸身份值分别为 72.6%、72.7%和 72.3%。与三个有效发表的 种的相关基因组序列相比，LNNU 331112 菌株的基因组序列显示出 69.0-72.3%的平均氨基酸身份值。LNNU 331112 菌株的基因组为 3.47 Mb，其 DNA G+C 含量为 68.4 mol%。在 LNNU 331112 菌株中鉴定出 3182 个基因作为蛋白质编码基因。基因组分析表明，LNNU 331112 菌株中存在许多与渗透压调节、细胞内 pH 稳态和钾（K）摄取蛋白相关的基因。主要的menaquinones 为 MK-8（44.6%）和 MK-7（55.4%），这将 LNNU 331112 菌株与其他三个公认的 种区分开来。主要脂肪酸（>10%）为 Cω8（33.8%）、C（23.3%）、C（12.8%）和总和特征 3（12.9%），这也将 LNNU 331112 菌株与其他三个公认的 种明显区分开来。LNNU 331112 菌株的极性脂谱包括二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、一种未鉴定的糖脂、一种未鉴定的磷脂和两种未鉴定的脂类。根据表型、化学分类和系统发育分析的结果，LNNU 331112 菌株被认为代表了 属的一个新种，因此提出了 sp. nov.的名称。该模式菌株为 LNNU 331112（=KCTC 39808=CGMCC 1.17107=DSM 103463）。
