The Key Laboratory of Plant Biotechnology of Liaoning Province, School of Life Science, Liaoning Normal University, Dalian, 650081, PR China.
Dandong Forestry and Grassland Development Service Center, Dandong 118000, PR China.
Int J Syst Evol Microbiol. 2023 Oct;73(10). doi: 10.1099/ijsem.0.006088.
Light yellowish-white colonies of a bacterial strain, designated LNNU 24178, were isolated from the rhizosphere soil of halophyte (Bunge) Freitag and Schütze grown at Shihezi district, Xinjiang, PR China. Cells were Gram-stain-negative, non-flagellum-forming, rod-shaped and non-motile. The results of phylogenetic analysis based on the 16S rRNA gene sequence indicated that LNNU 24178 represented a member of the genus and shared the highest sequence similarity with CGMCC 1.13927 (97.1 %) and lower sequence similarity (< 97.0 %) to other known species. The genomic DNA G+C content of LNNU 24178 was 68.8 %. The average nucleotide identity (ANI) values between LNNU 24178 and CGMCC 1.13927, DSM 12574, 26-35 and HB2 were 78.7, 78.6, 78.4 and 80.0 %, respectively. The digital DNA-DNA hybridisation (dDDH) values between LNNU 24178 and CGMCC 1.13927, DSM 12574, 26-35 and HB2 were 22.0, 22.3, 22.2 and 23.5 %, respectively. The respiratory quinone detected in LNNU 24178 was ubiquinone-8 (Q-8). The major fatty acids (> 5.0 %) of LNNU 24178 were identified as iso-C (33.9 %), iso-C (8.7 %), iso-C (6.2 %), iso-C (5.7 %), C (5.3 %) and summed feature 9 (iso-Cω9/10-methyl C) (21.1 %). The major polar lipids of LNNU 24178 were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), one unidentified phospholipid (PL), one unidentified glycolipid (GL) and three unidentified lipids. According to the data obtained from phenotypic, chemotaxonomic and phylogenetic analyses, strain LNNU 24178 represents a novel species of the genus , for which the name sp. nov. is proposed, with LNNU 24178 (= CGMCC 1.17331= KCTC 62251) as the type strain.
一株细菌菌株的淡黄色白色菌落，命名为 LNNU 24178，是从生长在中国新疆石河子地区盐生植物（Bunge）Freitag 和 Schütze 的根际土壤中分离出来的。细胞革兰氏染色阴性，无鞭毛，杆状，非运动性。基于 16S rRNA 基因序列的系统发育分析结果表明，LNNU 24178 是一个属的成员，与 CGMCC 1.13927（97.1%）的序列相似度最高，与其他已知种的序列相似度较低（<97.0%）。LNNU 24178 的基因组 DNA G+C 含量为 68.8%。LNNU 24178 与 CGMCC 1.13927、DSM 12574、26-35 和 HB2 的平均核苷酸身份（ANI）值分别为 78.7%、78.6%、78.4%和 80.0%。LNNU 24178 与 CGMCC 1.13927、DSM 12574、26-35 和 HB2 的数字 DNA-DNA 杂交（dDDH）值分别为 22.0%、22.3%、22.2%和 23.5%。LNNU 24178 中检测到的呼吸醌为泛醌-8（Q-8）。LNNU 24178 的主要脂肪酸（>5.0%）为 iso-C（33.9%）、iso-C（8.7%）、iso-C（6.2%）、iso-C（5.7%）、C（5.3%）和总和特征 9（iso-Cω9/10-甲基 C）（21.1%）。LNNU 24178 的主要极性脂为双磷脂酰甘油（DPG）、磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）、一种未鉴定的磷脂（PL）、一种未鉴定的糖脂（GL）和三种未鉴定的脂。根据表型、化学分类和系统发育分析获得的数据，菌株 LNNU 24178 代表了属的一个新种，建议将其命名为 sp. nov.，其中 LNNU 24178（=CGMCC 1.17331=KCTC 62251）作为模式菌株。
