Infanzón Belén, Herrmann Kevin R, Hofmann Isabell, Willbold Sabine, Ruff Anna Joëlle, Schwaneberg Ulrich
Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany.
Central Institute for Engineering, Electronics and Analytics, Analytics (ZEA-3), Wilhelm-Johnen-Straße, 52428 Jülich, Germany.
Enzyme Microb Technol. 2022 Jan;153:109953. doi: 10.1016/j.enzmictec.2021.109953. Epub 2021 Nov 23.
Phytases are hydrolytic enzymes capable of a stepwise phosphate release from phytate which is the main phosphorous storage in seeds, cereals and legumes. Limitations such as low enzyme activity or incomplete phytate hydrolysis to inositol are a great challenge in phytase applications in food and feed. Herein we report a phytase blend of two enzymes with additive effects on phytate (InsP6) hydrolysis and its application in the enzymatic phosphorous recovery process. Blending the fast 6-phytase rPhyXT52 with the 3-phytase from Debaryomyces castellii, which is capable of fully hydrolyzing InsP6, we achieved rapid phosphate release with higher yields compared to the individual enzymes and a rapid disappearance of InsP6-3 intermediates, monitored by HPLC. NMR data suggest a nearly complete phytate hydrolysis to inositol and phosphate. The blend was applied for phosphate mobilization from phytate-rich biomass, such as deoiled seeds. For this emerging application, an up to 43% increased phosphate mobilization yield was achieved when using 1000 U of the blend per kg biomass compared to using only the E. coli phytase. Even so, the time of enzyme treatment was decreased by more than half (6 h instead of 16 h) when using 4000 U of blend, we reached a 78-90% reduction of the total phosphorous content in the explored deoiled seeds. In summary, the phytase blend of Dc phyt/rPhyXT52 was proven very efficient to obtain inositol phosphate depleted meal which has its potential application in animal feeding and is concomitant with the production of green phosphate from renewable resources.
植酸酶是一种水解酶，能够逐步从植酸盐中释放磷酸盐，植酸盐是种子、谷物和豆类中主要的磷储存形式。诸如酶活性低或植酸盐不完全水解为肌醇等限制因素，是植酸酶在食品和饲料应用中的巨大挑战。在此，我们报告了一种由两种酶组成的植酸酶混合物，其对植酸盐（肌醇六磷酸）水解具有加和效应，并介绍了其在酶法磷回收过程中的应用。将快速作用的6-植酸酶rPhyXT52与来自卡氏德巴利酵母的3-植酸酶（能够完全水解肌醇六磷酸）混合，通过高效液相色谱监测，我们实现了比单独使用酶更高产率的快速磷酸盐释放以及肌醇六磷酸-3中间体的快速消失。核磁共振数据表明植酸盐几乎完全水解为肌醇和磷酸盐。该混合物用于从富含植酸盐的生物质（如脱油种子）中动员磷酸盐。对于这种新兴应用，与仅使用大肠杆菌植酸酶相比，每千克生物质使用1000 U该混合物时，磷酸盐动员产率提高了43%。即便如此，当使用4000 U混合物时，酶处理时间减少了一半以上（从16小时降至6小时），我们使所研究的脱油种子中的总磷含量降低了78 - 90%。总之，已证明卡氏德巴利酵母植酸酶/ rPhyXT52的植酸酶混合物在获得低肌醇磷酸盐粕方面非常有效，该粕在动物饲料中有潜在应用，同时伴随着从可再生资源中生产绿色磷酸盐。
