Ohadi Elnaz, Bakhshi Bita, Kalani Behrooz Sadeghi, Talebi Malihe, Irajian Gholamreza
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran; Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Res Vet Sci. 2021 Nov 19;142:24-30. doi: 10.1016/j.rvsc.2021.11.009.
It has been well documented that Campylobacter is the leading cause of foodborne infections and bacterial enteritis in high-income countries. The gastrointestinal tract of most warm-blooded animals, such as mammals and poultry, is prone to this pathogen. Infections caused by this bacterium in humans have usually been associated with the consumption of contaminated poultry meat. The important point about Campylobacter is that this bacterium has adapted to harsh environmental conditions along the food chain (poultry digestive tract to the consumer's plate) and developed an adapted mechanism to those conditions. This study aimed to compare the ability of Campylobacter jejuni and Campylobacter coli strains to form biofilms under aerobic and microaerobic conditions. The presence and expression of flab, FliS, DnaK, luxs, CsrA, Cj0688, and cosR genes involved in biofilm formation were investigated. Finally, the correlation between the biofilm forming ability of Campylobacter isolates and the presence/expression of selected genes has been explored. A significant correlation was observed between the presence and expression of some genes and the degree of biofilm formation in C. jejuni and C. coli isolates. A strong biofilm production was detected in strains harboring all selected genes with greater expression levels. The ability of C. jejuni and C. coli strains in biofilm formation is associated with the coordinated function and convergent expression of the selected genes. Seemingly, stress response- and motility-related genes have the most involvement in biofilm formation of C. jejuni and C. coli strains, while other genes have an accessory role in this phenomenon.
有充分文献记载，弯曲杆菌是高收入国家食源性感染和细菌性肠炎的主要病因。大多数温血动物的胃肠道，如哺乳动物和家禽，都易感染这种病原体。人类由这种细菌引起的感染通常与食用受污染的禽肉有关。关于弯曲杆菌的重要一点是，这种细菌已经适应了食物链（从家禽消化道到消费者餐盘）中的恶劣环境条件，并针对这些条件形成了一种适应机制。本研究旨在比较空肠弯曲杆菌和结肠弯曲杆菌菌株在需氧和微需氧条件下形成生物膜的能力。研究了参与生物膜形成的flab、FliS、DnaK、luxs、CsrA、Cj0688和cosR基因的存在及表达情况。最后，探讨了弯曲杆菌分离株的生物膜形成能力与所选基因的存在/表达之间的相关性。在空肠弯曲杆菌和结肠弯曲杆菌分离株中，观察到一些基因的存在和表达与生物膜形成程度之间存在显著相关性。在所有所选基因表达水平较高的菌株中检测到强烈的生物膜产生。空肠弯曲杆菌和结肠弯曲杆菌菌株的生物膜形成能力与所选基因的协同功能和趋同表达有关。似乎，应激反应和运动相关基因在空肠弯曲杆菌和结肠弯曲杆菌菌株的生物膜形成中参与最多，而其他基因在这一现象中起辅助作用。
