Hanash S M, Baier L J, McCurry L, Schwartz S A
Proc Natl Acad Sci U S A. 1986 Feb;83(3):807-11. doi: 10.1073/pnas.83.3.807.
We have utilized two-dimensional polyacrylamide gel electrophoresis, coupled with ultrasensitive silver staining, to identify lineage-related polypeptide markers in lymphoblasts from children with acute lymphoblastic leukemia. Twelve polypeptides were detected that could distinguish between the major subgroups of acute lymphoblastic leukemia. These included a new marker for common acute lymphoblastic leukemia and markers for cells of B and T lineages. Analysis of the two-dimensional patterns also allowed the tentative identification of T-cell lineage in two cases with an otherwise undifferentiated non-T-cell non-B-cell phenotype. Two-dimensional electrophoresis thus provides a powerful tool for the delineation of the cell of origin in leukemia.
我们利用二维聚丙烯酰胺凝胶电泳，并结合超灵敏银染技术，来鉴定急性淋巴细胞白血病患儿淋巴母细胞中与谱系相关的多肽标志物。检测到12种多肽，它们可区分急性淋巴细胞白血病的主要亚组。其中包括一种常见急性淋巴细胞白血病的新标志物以及B和T谱系细胞的标志物。对二维图谱的分析还使得在两例具有未分化非T细胞非B细胞表型的病例中初步鉴定出T细胞谱系。因此，二维电泳为白血病起源细胞的描绘提供了一个强大的工具。
