Bradstock K F, Janossy G, Pizzolo G, Hoffbrand A V, McMichael A, Pilch J R, Milstein C, Beverley P, Bollum F J
J Natl Cancer Inst. 1980 Jul;65(1):33-42.
Combinations of antibodies to membrane antigens and to terminal deoxynucleotidyl transferase (TdT) were used to study human thymocyte and bone marrow subpopulations and leukemia cells. Cortical thymocytes were TdT+ and expressed T-cell antigens (HuTLA+), a thymocyte-specific antigen (HTA-1+), and a leukocyte antigen (HLe-l++) but lacked detectable HLA-A,B,C and la (HLA-D) antigens. In contrast, medullary thymocytes were TdT-, HuTLA+, HTA-1-, HLe-l++. A small subpopulation of larger, probably immature, thymocytes were strongly TdT+, HuTLA+, la-, HTA-1-, HLe-l +/-. Many blast cells from cases of thymic acute lymphoblastic leukemia (Thy-ALL) showed the phenotype of this small subset, and only a proportion of Thy-ALL blast cells exhibited HTA-1 and HLe-l antigens as strongly as was observed on normal cortical thymocytes. In contrast, TdT+ cells observed in normal juvenile bone marrow were HuTLA, HTA-1-, HLA+, la+. This phenotype corresponded to the phenotype of the common form of ALL (non-T, non-B) and indicated that further studies are necessary to analyze the differentiation of bone marrow precursors to thymic cells.
利用针对膜抗原和末端脱氧核苷酸转移酶（TdT）的抗体组合来研究人类胸腺细胞、骨髓亚群和白血病细胞。皮质胸腺细胞为TdT阳性，并表达T细胞抗原（HuTLA阳性）、一种胸腺细胞特异性抗原（HTA-1阳性）和一种白细胞抗原（HLe-1强阳性），但缺乏可检测到的HLA-A、B、C和Ia（HLA-D）抗原。相比之下，髓质胸腺细胞为TdT阴性、HuTLA阳性、HTA-1阴性、HLe-1强阳性。一小部分较大的、可能未成熟的胸腺细胞为强TdT阳性、HuTLA阳性、Ia阴性、HTA-1阴性、HLe-1弱阳性。许多胸腺急性淋巴细胞白血病（Thy-ALL）病例的原始细胞表现出这一小部分细胞的表型，并且只有一部分Thy-ALL原始细胞表现出与正常皮质胸腺细胞上观察到的一样强的HTA-1和HLe-1抗原。相比之下，在正常青少年骨髓中观察到的TdT阳性细胞为HuTLA阴性、HTA-1阴性、HLA阳性、Ia阳性。这种表型与常见形式的急性淋巴细胞白血病（非T、非B）的表型相对应，表明有必要进一步研究以分析骨髓前体细胞向胸腺细胞的分化。
