Department of Environment and Health, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.
Department of Environment and Health, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.
Sci Total Environ. 2022 Feb 20;808:151739. doi: 10.1016/j.scitotenv.2021.151739. Epub 2021 Nov 27.
PFHxS (Perfluorohexane sulfonic acid) is one of the short-chain perfluoroalkyl substances (PFASs) which are widely used in many industrial and consumer applications. However, limited information is available on the molecular mechanism of PFHxS toxicity (e.g. lipid metabolism). This study provides in-depth information on the lipid regulation of zebrafish embryos with and without PFHxS exposure. Lipid changes throughout zebrafish development (4 to 120 h post fertilization (hpf)) were closely associated with lipid species and lipid composition (fatty acyl chains). A comprehensive lipid analysis of four different PFHxS exposures (0, 0.3, 1, 3, and 10 μM) at different zebrafish developmental stages (24, 48, 72, and 120 hpf) was performed. Data on exposure concentration, lipids, and developmental stage showed that all PFHxS concentrations dysregulated the lipid metabolism and these were developmental-dependent. The pattern of significantly changed lipids revealed that PFHxS caused effects related to oxidative stress, inflammation, and impaired fatty acid β-oxidation. Oxidative stress and inflammation caused the remodeling of glycerophospholipid (phosphatidylcholine (PC) and phosphatidylethanolamine (PE)), with increased incorporation of omega-3 PUFA and a decreased incorporation of omega-6 PUFA.
全氟己烷磺酸（PFHxS）是短链全氟烷基物质（PFASs）之一，广泛应用于许多工业和消费产品。然而，关于 PFHxS 毒性的分子机制（例如脂质代谢）的信息有限。本研究深入探讨了暴露于 PFHxS 前后斑马鱼胚胎的脂质调节。在斑马鱼发育过程中（受精后 4 至 120 小时（hpf）），脂质变化与脂质种类和脂质组成（脂肪酸链）密切相关。在不同的斑马鱼发育阶段（24、48、72 和 120 hpf）对四种不同的 PFHxS 暴露（0、0.3、1、3 和 10 μM）进行了全面的脂质分析。暴露浓度、脂质和发育阶段的数据表明，所有 PFHxS 浓度均使脂质代谢失调，且这种失调具有发育依赖性。显著变化的脂质模式表明，PFHxS 导致与氧化应激、炎症和脂肪酸β-氧化受损相关的影响。氧化应激和炎症导致甘油磷脂（磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE））的重塑，增加了 ω-3PUFA 的掺入，减少了 ω-6PUFA 的掺入。
