Albers Janice, Mylroie John, Kimble Ashley, Steward Catherine, Chapman Kacy, Wilbanks Mitchell, Perkins Edward, Garcia-Reyero Natàlia
Oak Ridge Institute for Science and Education, Environmental Laboratory, US Army Engineer Research & Development Center, Vicksburg, MS 39180, USA.
Environmental Laboratory, US Army Engineer Research & Development Center, Vicksburg, MS 39180, USA.
Toxics. 2024 Feb 29;12(3):192. doi: 10.3390/toxics12030192.
The presence of per- and polyfluoroalkyl substances (PFASs) in aquatic environments is often persistent and widespread. Understanding the potential adverse effects from this group of chemicals on aquatic communities allows for better hazard characterization. This study examines impacts on zebrafish () embryo physiology, behavior, and lipid levels from exposure to perfluorooctanoic acid (PFOA), perfluorohexane sulfonate (PFHxS), and heptadecafluorooctanesulfonic acid (PFOS). Embryos were exposed to lethal and sublethal levels of each chemical and monitored for alterations in physiological malformations, mortality, lipid levels, and behavior (only PFOA and PFHxS). The predicted 50% lethal concentrations for 120 hpf embryos were 528.6 ppm PFOA, 14.28 ppm PFHxS, and 2.14 ppm PFOS. Spine curvature and the inability of the 120 hpf embryos to maintain a dorsal-up orientation was significantly increased at 10.2 ppm PFHxS and 1.9 ppm PFOS exposure. All measured 120 hpf embryo behaviors were significantly altered starting at the lowest levels tested, 188 ppm PFOA and 6.4 ppm PFHxS. Lipid levels decreased at the highest PFAS levels tested (375 PFOA ppm, 14.4 PFHxS ppm, 2.42 ppm PFOS). In general, the PFAS chemicals, at the levels examined in this study, increased morphological deformities, embryo activity, and startle response time, as well as decreased lipid levels in 120 hpf zebrafish embryos.
水生环境中全氟和多氟烷基物质（PFASs）的存在往往具有持久性且分布广泛。了解这类化学物质对水生生物群落的潜在不利影响有助于更好地进行危害特征描述。本研究考察了暴露于全氟辛酸（PFOA）、全氟己烷磺酸（PFHxS）和十七氟辛烷磺酸（PFOS）对斑马鱼胚胎生理、行为和脂质水平的影响。将胚胎暴露于每种化学物质的致死和亚致死水平，并监测其生理畸形、死亡率、脂质水平和行为（仅针对PFOA和PFHxS）的变化。120小时胚胎的预测半数致死浓度分别为：PFOA 528.6 ppm、PFHxS 14.28 ppm和PFOS 2.14 ppm。暴露于10.2 ppm PFHxS和1.9 ppm PFOS时，120小时胚胎的脊柱弯曲以及无法保持背部向上的姿势显著增加。从测试的最低水平（PFOA 188 ppm和PFHxS 6.4 ppm）开始，所有测量的120小时胚胎行为均发生显著改变。在测试的最高PFAS水平（PFOA 375 ppm、PFHxS 14.4 ppm、PFOS 2.42 ppm）下，脂质水平下降。总体而言，在本研究考察的水平下，PFAS化学物质增加了120小时斑马鱼胚胎的形态畸形、胚胎活动和惊吓反应时间，并降低了脂质水平。
