Department of Internal Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Korea.
Department of Internal Medicine, Hanyang University Guri Hospital, Hanyang University College of Medicine, Guri, Korea.
J Nutr Biochem. 2022 Mar;101:108926. doi: 10.1016/j.jnutbio.2021.108926. Epub 2021 Nov 27.
Short-chain fatty acids (SCFAs) play a pivotal role in maintaining intestinal homeostasis. We aimed to investigate the effects of SCFA supplementation on gut inflammation and microbiota composition in a murine colitis model. Mice were fed with sodium butyrate or a mixture of SCFAs in the drinking water for 2 weeks, followed by 2% dextran sulfate sodium (DSS) for 7 d. After euthanasia, mouse colons were extracted to examine histological findings. Flow cytometry of the mouse colon tissues was performed to assess T cell differentiation. Changes in gut microbiota were assessed by high-throughput sequencing of the mouse feces. There were no significant differences in weight change, colonic length, or histologic inflammation score between the DSS, butyrate, and SCFA mix groups. However, flow cytometry revealed that both the expression of CD4+Foxp3+ regulatory T cells and of IL-17-producing T cells were increased in the butyrate and SCFA mix groups. Microbial compositions of the butyrate and SCFA mix groups were significantly different from those of the control and DSS groups in principal coordinate analysis. Relative abundances of the phyla Verrucomicrobia and Proteobacteria, species Akkermansia muciniphila and Escherichia fergusonii were increased in the butyrate and SCFA mix groups. Genera Roseburia and Lactobacillus showed a negative correlation with the degree of colitis, whereas genera Escherichia and Mucispirillum showed a positive correlation. SCFA supplementation did not result in a significant reduction in colon inflammation, but it promoted both regulatory T cell and IL-17-producing T cell expression, and increased both protective and aggressive gut microbiota.
短链脂肪酸（SCFAs）在维持肠道内环境稳定中起着关键作用。本研究旨在探究 SCFA 补充对结肠炎模型中肠道炎症和微生物组成的影响。将小鼠用丁酸钠或 SCFA 混合物通过饮用水进行喂养 2 周，随后用 2%葡聚糖硫酸钠（DSS）诱导 7 天。安乐死后，提取小鼠结肠以检查组织学发现。通过对小鼠结肠组织进行流式细胞术分析以评估 T 细胞分化。通过对小鼠粪便进行高通量测序来评估肠道微生物群的变化。DSS、丁酸钠和 SCFA 混合物组之间的体重变化、结肠长度或组织学炎症评分无显著差异。然而，流式细胞术显示丁酸钠和 SCFA 混合物组中 CD4+Foxp3+调节性 T 细胞和产生 IL-17 的 T 细胞的表达均增加。在主坐标分析中，丁酸钠和 SCFA 混合物组的微生物组成与对照组和 DSS 组有显著差异。厚壁菌门和变形菌门的相对丰度、阿克曼氏菌属和埃希氏菌属在丁酸钠和 SCFA 混合物组中增加。罗斯伯里氏菌属和乳杆菌属与结肠炎的严重程度呈负相关，而埃希氏菌属和黏液螺旋菌属呈正相关。SCFA 补充并未显著减轻结肠炎症，但它促进了调节性 T 细胞和产生 IL-17 的 T 细胞的表达，并增加了保护性和侵袭性肠道微生物群。
