Department of Parasitology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK.
Sci Rep. 2021 Nov 30;11(1):23181. doi: 10.1038/s41598-021-02388-8.
The endemic strain of Leishmania donovani in Sri Lanka causes cutaneous leishmaniasis (CL) rather than more common visceral form. We have visualized biofilms and profiled the microbiome of lesions and unaffected skin in thirty-nine CL patients. Twenty-four lesions (61.5%) were biofilm-positive according to fluorescence in situ hybridization. Biopsies of biofilm-positive lesions were dominated by Pseudomonas, class Bacilli and Enterobacteriaceae and distinguished by significantly lower community evenness. Higher relative abundance of a class Bacilli OTU was detected in wound swabs versus contralateral skin. Wound swabs and biopsies had significantly distinct microbiome profiles and lower diversity compared to unaffected skin. Greater abundances of potentially pathogenic organisms were observed in wet ulcers, lesions with high parasite loads and large wounds. In summary, more than half of L. donovani associated CL wounds harboured biofilms and the wounds exhibited a distinct, less diverse, microbiome than unaffected skin.
斯里兰卡地方性的杜氏利什曼原虫（Leishmania donovani）株引起皮肤利什曼病（cutaneous leishmaniasis，CL），而不是更为常见的内脏利什曼病。我们对 39 例 CL 患者的病变和未受影响的皮肤进行了生物膜可视化和微生物组分析。根据荧光原位杂交，有 24 处病变（61.5%）呈生物膜阳性。生物膜阳性病变的活检标本主要由假单胞菌、B 类杆菌和肠杆菌科组成，其群落均匀度显著降低。与对侧皮肤相比，在伤口拭子中检测到 B 类杆菌 OTU 的相对丰度更高。与未受影响的皮肤相比，伤口拭子和活检标本的微生物组谱明显不同，多样性也较低。在湿性溃疡、寄生虫负荷高和大伤口的病变中，观察到更多潜在的致病性生物体。总之，超过一半的与杜氏利什曼原虫相关的 CL 伤口存在生物膜，并且与未受影响的皮肤相比，伤口的微生物组具有明显不同、多样性较低的特征。
