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固定在经苯丙氨酸功能化的冷冻凝胶上的漆酶用于纺织染料的脱色。

Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes.

作者信息

Perçin Işık, Aracagök Yusuf Doruk, Idil Neslihan, Denizli Adil, Mattiasson Bo

机构信息

Department of Biology, Molecular Biology Division, Faculty of Science, Hacettepe University, Ankara Turkey.

Department of Biology, Biotechnology Division, Faculty of Science, Hacettepe University, Ankara Turkey.

出版信息

Turk J Chem. 2021 Oct 19;45(5):1353-1365. doi: 10.3906/kim-2106-16. eCollection 2021.

DOI:10.3906/kim-2106-16
PMID:34849053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8596530/
Abstract

In this study, amino acid functionalized poly(2-hydroxyethyl methacrylate-N-methacrylolyl-l-phenylalanine) [PHEMAPA] cryogel discs were prepared. In this respect, phenylalanine containing N-methacryloyl-(L)-phenylalanine methyl ester (MAPA) was polymerized with 2-hydroxyethyl methacrylate (HEMA) without requirement of any activation step. Laccase bound poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-phenylalanine) [Lac-PHEMAPA] cryogel discs were applied for decolorization of Reactive Blue-247 (RB-247). The ability of Lac-PHEMAPA cryogel discs on dye decolorization was found to be as 90% in 2 h and even more within 4h. The decolorization activities of 86% and 73% were observed at relatively low (4°C) and high (60°C) temperatures, respectively. The effect of dye concentration on dye decolorization and 100% decolorization activity was achieved in dye concentration between 50-300 ppm. Lac-PHEMAPA cryogel discs maintained 80% of its decolorization activity after six cycles. Consequently, the PHEMAPA cryogel discs are promising materials for immobilizing laccase. The Lac-PHEMAPA has a rapid dye decolorization in a broad range of temperature. The preparation is furthermore very stable and activity is preserved during storage.

摘要

在本研究中，制备了氨基酸功能化的聚（甲基丙烯酸2-羟乙酯-N-甲基丙烯酰基-L-苯丙氨酸）[PHEMAPA]冷冻凝胶盘。在这方面，含苯丙氨酸的N-甲基丙烯酰基-（L）-苯丙氨酸甲酯（MAPA）与甲基丙烯酸2-羟乙酯（HEMA）聚合，无需任何活化步骤。将漆酶结合的聚（甲基丙烯酸2-羟乙酯-N-甲基丙烯酰基-L-苯丙氨酸）[Lac-PHEMAPA]冷冻凝胶盘用于活性蓝-247（RB-247）的脱色。发现Lac-PHEMAPA冷冻凝胶盘对染料的脱色能力在2小时内可达90%，4小时内甚至更高。在相对较低（4°C）和较高（60°C）温度下，脱色活性分别为86%和73%。染料浓度对染料脱色有影响，在50-300 ppm的染料浓度范围内可实现100%的脱色活性。Lac-PHEMAPA冷冻凝胶盘在六个循环后仍保持其80%的脱色活性。因此，PHEMAPA冷冻凝胶盘是固定漆酶的有前景的材料。Lac-PHEMAPA在很宽的温度范围内具有快速的染料脱色能力。此外，该制剂非常稳定，在储存期间活性得以保留。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/24cabdc33d3b/turkjchem-45-1353-fig008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/9f7b207ddc82/turkjchem-45-1353-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/0f91f86486e2/turkjchem-45-1353-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/835f99553836/turkjchem-45-1353-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/ca8113c06707/turkjchem-45-1353-fig004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/702463068cfa/turkjchem-45-1353-fig004b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/d53a62fea96e/turkjchem-45-1353-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/7124394e03d7/turkjchem-45-1353-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/eab14f7823dd/turkjchem-45-1353-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/24cabdc33d3b/turkjchem-45-1353-fig008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/9f7b207ddc82/turkjchem-45-1353-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/0f91f86486e2/turkjchem-45-1353-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/835f99553836/turkjchem-45-1353-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/ca8113c06707/turkjchem-45-1353-fig004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/702463068cfa/turkjchem-45-1353-fig004b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/d53a62fea96e/turkjchem-45-1353-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/7124394e03d7/turkjchem-45-1353-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/eab14f7823dd/turkjchem-45-1353-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a04/8596530/24cabdc33d3b/turkjchem-45-1353-fig008.jpg

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Int J Biol Macromol. 2019 Feb 15;123:738-743. doi: 10.1016/j.ijbiomac.2018.11.121. Epub 2018 Nov 16.
2
Kinetic analysis and structural studies of a high-efficiency laccase from sp. RSD1.来自sp. RSD1的高效漆酶的动力学分析与结构研究。
FEBS Open Bio. 2018 Jul 3;8(8):1230-1246. doi: 10.1002/2211-5463.12459. eCollection 2018 Aug.
3
Laccase Activity and Azo Dye Decolorization Potential of .
漆酶活性与……的偶氮染料脱色潜力
Mycobiology. 2018 Mar 28;46(1):79-83. doi: 10.1080/12298093.2018.1454006. eCollection 2018.
4
Denaturation process of laccase in various media by refractive index measurements.通过折射率测量研究漆酶在各种介质中的变性过程。
Biochem Biophys Rep. 2017 May 31;11:19-26. doi: 10.1016/j.bbrep.2017.05.005. eCollection 2017 Sep.
5
Ammonium-Functionalized Hollow Polymer Particles As a pH-Responsive Adsorbent for Selective Removal of Acid Dye.铵功能化空心聚合物粒子作为 pH 响应吸附剂用于选择性去除酸性染料。
ACS Appl Mater Interfaces. 2016 Jul 6;8(26):16690-8. doi: 10.1021/acsami.6b04199. Epub 2016 Jun 23.
6
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Artif Cells Nanomed Biotechnol. 2016 Nov;44(7):1708-13. doi: 10.3109/21691401.2015.1089252. Epub 2015 Oct 27.
7
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Prep Biochem Biotechnol. 2016 Aug 17;46(6):602-9. doi: 10.1080/10826068.2015.1085400.
8
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J Environ Health Sci Eng. 2015 Apr 25;13:38. doi: 10.1186/s40201-015-0192-0. eCollection 2015.
9
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J Hazard Mater. 2015 Jul 15;292:52-60. doi: 10.1016/j.jhazmat.2015.03.018. Epub 2015 Mar 11.
10
Decolorization of anthraquinonic dyes from textile effluent using horseradish peroxidase: optimization and kinetic study.使用辣根过氧化物酶对纺织废水中蒽醌染料进行脱色：优化与动力学研究
ScientificWorldJournal. 2015;2015:371625. doi: 10.1155/2015/371625. Epub 2015 Jan 19.