Göktürk Ilgım, Perçin Işık, Denizli Adil
a Biochemistry Division, Department of Chemistry , Hacettepe University , Beytepe , Ankara , Turkey.
b Nanotechnology and Nanomedicine Division , Institute of Science, Hacettepe University , Beytepe , Ankara , Turkey.
Prep Biochem Biotechnol. 2016 Aug 17;46(6):602-9. doi: 10.1080/10826068.2015.1085400.
In this study, iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) (PHEMAGA/Fe(3+)) cryogel discs were prepared. The PHEMAGA/Fe(3+) cryogel discs were characterized by elemental analysis, scanning electron microscopy, Fourier transform infrared spectroscopy, swelling tests, and surface area measurements. The PHEMAGA/Fe(3+) cryogel discs had large pores ranging from 10 to 100 µm with a swelling degree of 9.36 g H2O/g cryogel. Effects of pH, temperature, initial catalase concentration, and flow rate on adsorption capacity of the PHEMAGA/Fe(3+) cryogel discs were investigated. Maximum catalase adsorption capacity (62.6 mg/g) was obtained at pH 7.0, 25°C, and 3 mg/ml initial catalase concentration. The PHEMAGA/Fe(3+) cryogel discs were also tested for the purification of catalase from rat liver. After tissue homogenization, purification of catalase was performed using the PHEMAGA/Fe(3+) cryogel discs and catalase was obtained with a yield of 54.34 and 16.67 purification fold.
在本研究中，制备了铁螯合的聚（甲基丙烯酸羟乙酯-N-甲基丙烯酰基-（l）-谷氨酸）（PHEMAGA/Fe(3+)）冷冻凝胶盘。通过元素分析、扫描电子显微镜、傅里叶变换红外光谱、溶胀试验和表面积测量对PHEMAGA/Fe(3+)冷冻凝胶盘进行了表征。PHEMAGA/Fe(3+)冷冻凝胶盘具有10至100μm的大孔，溶胀度为9.36g H2O/g冷冻凝胶。研究了pH、温度、初始过氧化氢酶浓度和流速对PHEMAGA/Fe(3+)冷冻凝胶盘吸附容量的影响。在pH 7.0、25°C和3mg/ml初始过氧化氢酶浓度下获得了最大过氧化氢酶吸附容量（62.6mg/g）。还测试了PHEMAGA/Fe(3+)冷冻凝胶盘从大鼠肝脏中纯化过氧化氢酶的能力。组织匀浆后，使用PHEMAGA/Fe(3+)冷冻凝胶盘进行过氧化氢酶的纯化，获得的过氧化氢酶产率为54.34，纯化倍数为16.67。
