Zhao Qianhua, Yang Qiya, Wang Zhenshuo, Sui Yuan, Wang Qi, Liu Jia, Zhang Hongyin
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
Department of Plant Pathology, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Comput Struct Biotechnol J. 2021 Oct 4;19:5589-5599. doi: 10.1016/j.csbj.2021.09.037. eCollection 2021.
Biological control utilizing antagonistic yeasts is an effective method for controlling postharvest diseases. Long non-coding RNAs (lncRNAs) have been found to be involved in a variety of plant growth and development processes, including those associated with plant disease resistance. In the present study, the yeast antagonist, , was found to strongly inhibit postharvest blue mold () and gray mold () decay of kiwifruit. Additionally, lncRNA high-throughput sequencing and bioinformatic analysis was used to identify lncRNAs in -treated wounds in kiwifruit and predict their function based on putative target genes. Our results indicate that lncRNAs may be involved in increasing ethylene (ET), jasmonic acid (JA), abscisic acid (ABA), and auxin (IAA) levels, as well as activating signal transduction pathways that regulate the expression of several transcription factors (, , ). These transcription factors (TFs) then mediate the expression of downstream, defense-related genes (, , ) and the synthesis of secondary metabolites, thus, potentially enhancing disease resistance. Notably, by stimulating the accumulation of antifungal compounds, such as phenols and lignin, disease resistance in kiwifruit was enhanced. Our study provides new information on the mechanism underlying the induction of disease resistance in kiwifruit by , as well as a new disease resistance strategy that can be used to enhance the defense response of fruit to pathogenic fungi.
利用拮抗菌控制采后病害是一种有效的方法。长链非编码RNA（lncRNAs）已被发现参与多种植物生长和发育过程，包括与植物抗病性相关的过程。在本研究中，发现酵母拮抗菌能强烈抑制猕猴桃采后的青霉病和灰霉病腐烂。此外，利用lncRNA高通量测序和生物信息学分析来鉴定猕猴桃中经拮抗菌处理的伤口中的lncRNAs，并根据推定的靶基因预测其功能。我们的结果表明，lncRNAs可能参与提高乙烯（ET）、茉莉酸（JA）、脱落酸（ABA）和生长素（IAA）水平，以及激活调节几种转录因子（、、）表达的信号转导途径。这些转录因子（TFs）随后介导下游防御相关基因（、、）的表达和次生代谢产物的合成，从而潜在地增强抗病性。值得注意的是，通过刺激酚类和木质素等抗真菌化合物的积累，增强了猕猴桃的抗病性。我们的研究为酵母拮抗菌诱导猕猴桃抗病性的潜在机制提供了新信息，以及一种可用于增强果实对致病真菌防御反应的新抗病策略。
