诱导斑马鱼幼虫上皮细胞丢失后真菌感染的实验方案。

Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

STAR Protoc. 2021 Nov 19;2(4):100963. doi: 10.1016/j.xpro.2021.100963. eCollection 2021 Dec 17.

Epithelia provide the first line of defense against foreign pathogens, and disruption of tissue homeostasis frequently allows for opportunistic infections. Here we provide a protocol for induction of epithelial cell loss in zebrafish larvae, followed by infection with fungal pathogens. Details are provided for monitoring larval survival after infection, assessment of fungal burden, and prophylactic treatment with antifungal compounds. Limitations of the protocol include potential antifungal toxicity and high fungal inoculums to induce lethal infection with some pathogenic fungal species. For complete details on the use and execution of this protocol, please refer to Wurster et al. (2021).

上皮组织为抵御外来病原体提供了第一道防线，而组织平衡的破坏常常会导致机会性感染。在这里，我们提供了一种在斑马鱼幼虫中诱导上皮细胞丢失的方案，随后进行真菌病原体感染。方案中详细介绍了感染后监测幼虫存活率、评估真菌负荷以及用抗真菌化合物进行预防性治疗的方法。该方案存在一些局限性，例如潜在的抗真菌毒性和高真菌接种量，这可能会导致某些致病性真菌物种的致死性感染。如需了解本方案的使用和实施的完整详细信息，请参考 Wurster 等人（2021 年）的研究。