Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Genetics and Epigenetics Graduate Program, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX, USA.
STAR Protoc. 2021 Jun 12;2(2):100600. doi: 10.1016/j.xpro.2021.100600. eCollection 2021 Jun 18.
Cell elimination by extrusion is important for epithelial homeostasis, but knowing when and where cells will extrude has made studies difficult. Here, we describe a step-by-step protocol for inducing cell extrusion from the larval zebrafish epidermis. We detail how to capture the dynamics of extrusion via time-lapse imaging and describe how existing protocols can be implemented for the analysis of cell shape changes preceding extrusion events and derivation of mechanical measurements associated with these shape changes. For complete details on the use and execution of this protocol, please refer to Atieh et al. (2021).
细胞挤出是上皮组织稳态的重要过程，但要了解何时以及哪些细胞会发生挤出，这给研究带来了困难。在这里，我们描述了一个从斑马鱼幼虫表皮诱导细胞挤出的逐步方案。我们详细介绍了如何通过延时成像来捕获挤出的动态，并描述了如何实施现有的方案来分析挤出事件之前的细胞形状变化，并得出与这些形状变化相关的力学测量值。如需了解本方案使用和执行的完整详细信息，请参考 Atieh 等人（2021 年）的研究。
