Watanabe Mai, Sianoya Abraham, Mishima Riko, Therdtatha Phatthanaphong, Rodriguez Abigail, Ramos Donna Christene, Lee Yuan Kun, Dalmacio Leslie Michelle, Nakayama Jiro
Laboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Department of Biochemistry and Molecular Biology (DBMB), College of Medicine, University of the Philippines Manila, 547 Pedro Gil Street, Ermita, Manila 1000 Philippines.
FEMS Microbiol Lett. 2021 Dec 7;368(20). doi: 10.1093/femsle/fnab149.
Here, we aim to understand the condition of the gut microbiome of Filipino adults in relation to their diet and metabolic status. Compared to rural Albay (n = 67), the gut microbiome of subjects living in urban Manila (n = 25) was more colonized by the order Clostridiales, which was negatively correlated with host carbohydrate consumption. Principal component analysis using the genus composition of the 92 total subjects indicated four microbiome types: one type driven by Prevotella, which was associated with high rice consumption and mainly consisted of healthy Albay subjects, one Clostridiales-driven group containing a number of type 2 diabetes mellitus (T2D) subjects from both Manila and Albay who showed lower butyrate levels in association with a decrease in Mediterraneibacter faecis, and the other two types showing dysbiosis-like microbiomes with Lactobacillus and Bifidobacterium overgrowth, with a high ratio of T2D and obese subjects. Multivariate logistic regression analysis suggested high dietary energy intake, and two Veillonellaeae genera, Dialister and Megasphaera, as T2D risk factors, while Prevotella and M. faecis as anti-T2D factors. In conclusion, low-carbohydrate diets restructured the Prevotella-driven gut microbiome, which may predispose Filipino people with high energy diet to T2D.
在此，我们旨在了解菲律宾成年人肠道微生物群的状况与其饮食和代谢状态之间的关系。与阿尔拜省农村地区（n = 67）相比，居住在马尼拉市（n = 25）的受试者肠道微生物群中梭菌目定殖更多，这与宿主碳水化合物消耗呈负相关。使用92名受试者的属组成进行主成分分析表明有四种微生物群类型：一种由普氏菌驱动，与高大米摄入量相关，主要由健康的阿尔拜省受试者组成；一个由梭菌目驱动的组，包含来自马尼拉和阿尔拜省的一些2型糖尿病（T2D）受试者，这些受试者的丁酸盐水平较低，同时粪便地中海杆菌减少；另外两种类型表现为类似失调的微生物群，乳酸杆菌和双歧杆菌过度生长，T2D和肥胖受试者比例较高。多变量逻辑回归分析表明，高膳食能量摄入以及韦荣氏菌科的两个属，即戴阿利斯特菌属和巨球形菌属，是T2D的危险因素，而普氏菌属和粪便地中海杆菌是抗T2D因素。总之，低碳水化合物饮食会重塑由普氏菌驱动的肠道微生物群，这可能使高能量饮食的菲律宾人易患T2D。
