Kulagina N N, Ivanovskaia N D, Taranova I A, Kul'berg A Ia
Biull Eksp Biol Med. 1986 Feb;101(2):185-7.
Biosynthesis of C1q complement component by resident peritoneal macrophages from (CBA X C57BL/6)F1 mice has been studied in in vitro experiments. Using anti-mouse C1q antibodies immobilized on CNBr Sepharose it has been demonstrated that 14C glycine incorporates both into intracellular C1q and C1q secreted into the medium. The maximum radioactivity of intracellular C1q was observed 48 h after cultivation, with it dropping drastically between hours 72-96. Kinetics of radiolabelled C1q was similar, but 24 hours delayed. Cell viability during 96 h of cultivation remained unchanged. These data can be considered as the indication of feedback regulation of C1q biosynthesis at the cellular level.
通过体外实验研究了（CBA×C57BL/6）F1小鼠腹腔常驻巨噬细胞中C1q补体成分的生物合成。使用固定在溴化氰琼脂糖上的抗小鼠C1q抗体已证明，14C甘氨酸既掺入细胞内C1q，也掺入分泌到培养基中的C1q。培养48小时后观察到细胞内C1q的最大放射性，在72 - 96小时之间急剧下降。放射性标记的C1q的动力学相似，但延迟了24小时。培养96小时期间细胞活力保持不变。这些数据可被视为细胞水平上C1q生物合成反馈调节的指标。
