Drexler H G, Menon M, Sagawa K, Tatsumi E, Koshiba H, Koishi T, Minato K, Sugimoto T, Saito M, Morita M
Blut. 1986 Feb;52(2):99-109. doi: 10.1007/BF00321072.
1255 cases of leukemia-lymphoma were tested between 1972 and 1984 by multiple marker analysis. Routine leukemia phenotyping was performed using standard morphological and cytochemical techniques in combination with clinical and histo-pathological information; the main emphasis was put on immunological surface marker analysis using erythrocyte rosette assays, TdT and a large panel of poly- and monoclonal antibody tests. The 1255 cases were divided into these major types and subtypes: 349 cases of ALL and related immature T- and Burkitt-lymphomas (cALL, pre B-ALL, B-ALL and Burkitt-lymphomas, T-ALL and immature, mostly leukemic T-lymphomas, Null-ALL), 454 cases of mature T- and B-cell malignancies (T-CLL, mycosis fungoides, Sezary-syndrome, T-lymphomas, B-CLL, hairy cell leukemia, multiple myeloma, B-lymphomas), 263 cases of acute myeloid leukemias (AML, AMMoL/AMoL), 182 cases of chronic myeloid leukemias (CML in chronic phase, CMoL, CML in blast crisis), 6 cases of erythroleukemia and 1 case of megakaryoblastic leukemia. A simplified classification scheme which has been used in our laboratories is presented. Phenotyping is of diagnostic, prognostic and therapeutic relevance, most evidently for patients with ALL. Routine leukemia phenotyping should be performed with highly standardized techniques and reagents and by combining information from several fields in the multiple marker analysis. New areas of leukemia research might become very useful for the routine procedure of phenotyping.
1972年至1984年间，对1255例白血病 - 淋巴瘤患者进行了多标记分析检测。采用标准形态学和细胞化学技术结合临床及组织病理学信息进行常规白血病表型分析；主要重点是使用红细胞玫瑰花结试验、末端脱氧核苷酸转移酶（TdT）以及大量多克隆和单克隆抗体检测进行免疫表面标记分析。这1255例患者被分为以下主要类型和亚型：349例急性淋巴细胞白血病（ALL）及相关未成熟T细胞和伯基特淋巴瘤（cALL、前B - ALL、B - ALL和伯基特淋巴瘤、T - ALL以及未成熟的、主要为白血病性的T淋巴瘤、裸细胞ALL），454例成熟T细胞和B细胞恶性肿瘤（T - CLL、蕈样肉芽肿、Sezary综合征、T淋巴瘤、B - CLL、毛细胞白血病、多发性骨髓瘤、B淋巴瘤），263例急性髓细胞白血病（AML、急性粒单核细胞白血病/急性单核细胞白血病），182例慢性髓细胞白血病（慢性期CML、慢性粒单核细胞白血病、急变期CML），6例红白血病和1例巨核细胞白血病。本文介绍了我们实验室使用的简化分类方案。表型分析具有诊断、预后和治疗相关性，对ALL患者最为明显。常规白血病表型分析应采用高度标准化的技术和试剂，并结合多标记分析中多个领域的信息。白血病研究的新领域可能对表型分析的常规程序非常有用。
