Holland M L, Ng K T
J Chromatogr. 1986 Jan 10;374(1):87-93. doi: 10.1016/s0378-4347(00)83255-7.
A highly sensitive and specific capillary gas chromatographic method for the determination of the antianginal drug bepridil in plasma is described. The capillary gas chromatograph and nitrogen-selective detector combination provides excellent sensitivity for clinical samples. The lowest concentration of bepridil which can be measured accurately and precisely in a 1-ml plasma sample is 1 ng/ml. Standard curves are linear over the concentration range 1-60 ng/ml. Accuracy and precision of the assay, expressed as relative deviation from the true value and relative standard deviation (inter-run) are less than 15% at all concentrations in the linear range. No interfering peaks are observed. Using an automatic injector and a laboratory computer system, sixty samples can be analyzed routinely in one day. The present assay has been successfully cross-validated with a published high-performance liquid chromatographic assay.
本文描述了一种用于测定血浆中抗心绞痛药物苄普地尔的高灵敏度和特异性的毛细管气相色谱法。毛细管气相色谱仪与氮选择性检测器联用，对临床样本具有出色的灵敏度。在1 ml血浆样本中能够准确精密测定的苄普地尔最低浓度为1 ng/ml。标准曲线在1 - 60 ng/ml浓度范围内呈线性。该测定方法的准确度和精密度，以相对于真实值的相对偏差和相对标准偏差（批间）表示，在线性范围内的所有浓度下均小于15%。未观察到干扰峰。使用自动进样器和实验室计算机系统，一天可常规分析60个样本。本测定方法已与已发表的高效液相色谱法成功进行了交叉验证。
