高效液相色谱法测定生物流体中的苄普地尔

Determination of bepridil in biological fluids by high-performance liquid chromatography.

作者信息

Ng K T, Plutte J A, Galante L J

出版信息

J Chromatogr. 1984 Jul 13;309(1):125-31. doi: 10.1016/0378-4347(84)80012-2.

Abstract

A rapid, selective and sensitive assay has been developed for the determination of the anti-anginal drug, bepridil, in biological samples. The lowest concentration of bepridil which can be measured accurately and precisely in a 2-ml plasma or urine sample is 10 ng/ml. The standard curve is linear in the concentration range 10-2000 ng/ml. Accuracy and precision of the assay, expressed as relative deviation and coefficient of variation (inter-run) are less than 6.5% at all concentrations in the linear range. No interfering peaks are observed. Using an automatic injector and a laboratory computer system, 48 samples are analyzed routinely in an 8-h day.

摘要

已开发出一种快速、选择性和灵敏的分析方法，用于测定生物样品中的抗心绞痛药物苄普地尔。在2 ml血浆或尿液样品中能够准确、精确测量的苄普地尔最低浓度为10 ng/ml。标准曲线在10 - 2000 ng/ml浓度范围内呈线性。该分析方法的准确度和精密度，以相对偏差和变异系数（批间）表示，在线性范围内的所有浓度下均小于6.5%。未观察到干扰峰。使用自动进样器和实验室计算机系统，每天8小时可常规分析48个样品。