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转录因子通过在苹果采后贮藏过程中与启动子结合参与淀粉-糖代谢。

Transcription Factor Participates in Starch-Sugar Metabolism by Binding to the Promoter in Apples During Postharvest Storage.

机构信息

Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.

出版信息

J Agric Food Chem. 2021 Dec 15;69(49):14906-14914. doi: 10.1021/acs.jafc.1c03343. Epub 2021 Dec 1.

Abstract

Starch degradation with fruit ripening is closely related to the aging process and flavor formation in apples. In this study, ethylene, 1-methylcyclopropene (1-MCP), and apples treated at different temperatures were used to determine the key genes of starch-sugar metabolism during storage. Compared with 4 °C storage, 20 °C storage promoted starch degradation and sugar accumulation in apples. In addition, ethylene treatment promoted starch degradation and sugar accumulation in apples, while 1-MCP treatment showed the opposite effects. The expression of indicated the crucial role of in starch-sugar conversion. Transient overexpression of significantly reduced the starch content in apples. Furthermore, directly combined the promoter and activated the expression, which may promote the starch degradation in apples. Therefore, it was concluded that may be involved in the regulation of starch-sugar metabolism in postharvest apples by activating the expression.

摘要

淀粉降解与果实成熟衰老密切相关,也与苹果风味形成有关。本研究以乙烯、1-甲基环丙烯(1-MCP)及不同温度处理的苹果为试材,探讨贮藏过程中淀粉-糖代谢关键基因的变化。与 4℃贮藏相比,20℃贮藏促进了苹果中淀粉的降解和糖的积累。乙烯处理促进了苹果中淀粉的降解和糖的积累,而 1-MCP 处理则表现出相反的效果。 的表达表明其在淀粉-糖转化中的关键作用。 的瞬时过表达显著降低了苹果中的淀粉含量。此外, 直接结合 启动子并激活 的表达,可能促进苹果中淀粉的降解。因此,推测 可能通过激活 的表达参与调控采后苹果的淀粉-糖代谢。

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