Escuela de Agronomía, Facultad de Ciencias Agronómicas y de los Alimentos, Pontificia Universidad Católica de Valparaíso, Quillota, Chile.
Departamento de Fruticultura y Enología, Facultad de Agronomía y Sistemas Naturales, Pontificia Universidad Católica de Chile, Santiago, Chile.
Biol Res. 2024 Sep 6;57(1):63. doi: 10.1186/s40659-024-00539-5.
Chilean peach growers have achieved worldwide recognition for their high-quality fruit products. Among the main factors influencing peach fruit quality, sweetness is pivotal for maintaining the market's competitiveness. Numerous studies have been conducted in different peach-segregating populations to unravel SSC regulation. However, different cultivars may also have distinct genetic conformation, and other factors, such as environmental conditions, can significantly impact SSC. Using a transcriptomic approach with a gene co-expression network analysis, we aimed to identify the regulatory mechanism that controls the sugar accumulation process in an 'O × N' peach population. This population was previously studied through genomic analysis, associating LG5 with the genetic control of the SSC trait. The results obtained in this study allowed us to identify 91 differentially expressed genes located on chromosome 5 of the peach genome as putative new regulators of sugar accumulation in peach, together with a regulatory network that involves genes directly associated with sugar transport (PpSWEET15), cellulose biosynthesis (PpCSLG2), flavonoid biosynthesis (PpPAL1), pectin modifications (PpPG, PpPL and PpPMEi), expansins (PpEXPA1 and PpEXPA8) and several transcription factors (PpC3H67, PpHB7, PpRVE1 and PpCBF4) involved with the SSC phenotype. These results contribute to a better understanding of the genetic control of the SSC trait for future breeding programs in peaches.
智利桃种植者因其高质量的水果产品而在世界范围内获得认可。在影响桃果实品质的诸多因素中，甜度对于保持市场竞争力至关重要。已经在不同的桃分离群体中进行了大量研究，以揭示 SSC 的调控。然而，不同的品种可能也有不同的遗传结构，其他因素，如环境条件，也会对 SSC 产生显著影响。本研究采用转录组学方法结合基因共表达网络分析，旨在鉴定控制 'O × N' 桃群体中糖积累过程的调控机制。该群体之前通过基因组分析进行了研究，将 LG5 与 SSC 性状的遗传控制联系起来。本研究的结果使我们能够鉴定出 91 个差异表达基因，这些基因位于桃基因组的第 5 号染色体上，可能是桃中糖积累的新调控因子，同时还构建了一个涉及与糖转运直接相关的基因（PpSWEET15）、纤维素生物合成（PpCSLG2）、类黄酮生物合成（PpPAL1）、果胶修饰（PpPG、PpPL 和 PpPMEi）、扩展蛋白（PpEXPA1 和 PpEXPA8）和几个转录因子（PpC3H67、PpHB7、PpRVE1 和 PpCBF4）的调控网络，这些基因与 SSC 表型有关。这些结果有助于更好地了解 SSC 性状的遗传控制，为未来的桃育种计划提供参考。
