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高度纯化的大鼠和人大颗粒淋巴细胞（LGL）中T细胞受体的分析：缺乏功能性1.3 kbβ链mRNA。

Analysis of T cell receptors in highly purified rat and human large granular lymphocytes (LGL): lack of functional 1.3 kb beta-chain mRNA.

作者信息

Young H A, Ortaldo J R, Herberman R B, Reynolds C W

出版信息

J Immunol. 1986 Apr 1;136(7):2701-4.

PMID:3485153
Abstract

Recent reports have suggested that the T cell receptor for antigen is somehow involved in the cytotoxicity of natural killer (NK) cells. However, we now report that highly purified, freshly isolated large granular lymphocytes (LGL) from both the human and rat, as well as LGL cultured in the presence of recombinant IL 2, express only the 1.0 kb beta-chain mRNA. The lack of a 1.3 kb mRNA, indicative of a functional beta-chain rearrangement, strongly suggests that a functional T cell receptor beta-chain is absent in freshly isolated LGL, thus making it extremely unlikely that this molecule is involved in target cell recognition by NK cells. These results also suggest that LGL are derived from a lineage distinct from T cells or develop before a functional rearrangement of the T cell receptor beta-chain.

摘要

最近的报告表明，抗原T细胞受体以某种方式参与自然杀伤（NK）细胞的细胞毒性作用。然而，我们现在报告，从人和大鼠中高度纯化、新鲜分离的大颗粒淋巴细胞（LGL），以及在重组白细胞介素2存在下培养的LGL，仅表达1.0 kb的β链mRNA。缺乏指示功能性β链重排的1.3 kb mRNA，强烈表明新鲜分离的LGL中不存在功能性T细胞受体β链，因此极不可能该分子参与NK细胞对靶细胞的识别。这些结果还表明，LGL源自与T细胞不同的谱系，或在T细胞受体β链功能性重排之前发育。

相似文献

1
Analysis of T cell receptors in highly purified rat and human large granular lymphocytes (LGL): lack of functional 1.3 kb beta-chain mRNA.高度纯化的大鼠和人大颗粒淋巴细胞（LGL）中T细胞受体的分析：缺乏功能性1.3 kbβ链mRNA。
J Immunol. 1986 Apr 1;136(7):2701-4.
2
Murine natural killer cells do not express functional transcripts of the alpha-, beta-, or gamma-chain genes of the T cell receptor.小鼠自然杀伤细胞不表达T细胞受体α链、β链或γ链基因的功能性转录本。
J Immunol. 1986 Nov 1;137(9):2998-3001.
3
Methylation patterns of the T cell receptor beta-chain gene in T cells, large granular lymphocytes, B cells, and monocytes.T细胞、大颗粒淋巴细胞、B细胞和单核细胞中T细胞受体β链基因的甲基化模式。
J Immunol. 1988 Jan 15;140(2):654-60.
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Effects of natural and recombinant IL 2 on regulation of IFN gamma production and natural killer activity: lack of involvement of the Tac antigen for these immunoregulatory effects.天然和重组白细胞介素2对γ干扰素产生调节及自然杀伤活性的影响：Tac抗原不参与这些免疫调节作用。
J Immunol. 1984 Aug;133(2):779-83.
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Murine natural killer cells stimulated in vivo do not express the T cell receptor alpha, beta, gamma, T3 delta, or T3 epsilon genes.体内受刺激的小鼠自然杀伤细胞不表达T细胞受体α、β、γ、T3δ或T3ε基因。
J Immunol. 1987 Sep 1;139(5):1704-10.
6
Limiting dilution analysis of the frequency of human T cells and large granular lymphocytes proliferating in response to interleukin 2. I. The effect of lectin on the proliferative frequency and cytotoxic activity of cultured lymphoid cells.限制稀释分析法测定人T细胞和大颗粒淋巴细胞对白介素2反应的增殖频率。I. 凝集素对培养淋巴细胞增殖频率和细胞毒性活性的影响。
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Chemotactic effect of human recombinant interleukin 2 on mouse activated large granular lymphocytes.人重组白细胞介素2对小鼠活化大颗粒淋巴细胞的趋化作用。
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T cell receptor genes do not rearrange or express functional transcripts in natural killer cells of scid mice.T细胞受体基因在严重联合免疫缺陷（scid）小鼠的自然杀伤细胞中不会重排或表达功能性转录本。
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Lysis of fresh human tumor cells by autologous large granular lymphocytes and T-lymphocytes: two distinct killing activities induced by coculture with autologous tumor.自体大颗粒淋巴细胞和T淋巴细胞对新鲜人肿瘤细胞的裂解作用：与自体肿瘤共培养诱导的两种不同杀伤活性。
J Natl Cancer Inst. 1984 Dec;73(6):1285-92.

引用本文的文献

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A cyclophilin-related protein involved in the function of natural killer cells.一种与亲环素相关的蛋白质，参与自然杀伤细胞的功能。
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):542-6. doi: 10.1073/pnas.90.2.542.
2
An expanded population of natural killer cells in mice with severe combined immunodeficiency (SCID) lack rearrangement and expression of T cell receptor genes.严重联合免疫缺陷（SCID）小鼠中自然杀伤细胞群体扩大，缺乏T细胞受体基因的重排和表达。
J Exp Med. 1986 Nov 1;164(5):1797-802. doi: 10.1084/jem.164.5.1797.
3
Specific inhibition of natural killer (NK) activity against different alloantigens.
对不同同种异体抗原的自然杀伤（NK）活性的特异性抑制。
Immunogenetics. 1987;26(6):329-38. doi: 10.1007/BF00343700.
4
T cell receptor gene rearrangements in cells with natural killer activity in the mouse.小鼠中具有自然杀伤活性的细胞中的T细胞受体基因重排。
Immunol Res. 1986;5(3):191-200. doi: 10.1007/BF02919200.
5
Inositol trisphosphate is generated by a rat natural killer cell tumor in response to target cells or to crosslinked monoclonal antibody OX-34: possible signaling role for the OX-34 determinant during activation by target cells.肌醇三磷酸由大鼠自然杀伤细胞肿瘤产生，以响应靶细胞或交联的单克隆抗体OX-34：OX-34决定簇在靶细胞激活过程中可能的信号传导作用。
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4239-43. doi: 10.1073/pnas.84.12.4239.
6
Human T-cell-receptor delta chain: genomic organization, diversity, and expression in populations of cells.人类T细胞受体δ链：细胞群体中的基因组组织、多样性及表达
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9714-8. doi: 10.1073/pnas.85.24.9714.
7
Rearrangement and expression of T cell receptor genes in cloned murine natural suppressor cell lines.克隆的小鼠自然抑制细胞系中T细胞受体基因的重排与表达
J Exp Med. 1987 Oct 1;166(4):1168-73. doi: 10.1084/jem.166.4.1168.
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Transplantable progenitors of natural killer cells are distinct from those of T and B lymphocytes.自然杀伤细胞的可移植祖细胞与T淋巴细胞和B淋巴细胞的可移植祖细胞不同。
Proc Natl Acad Sci U S A. 1986 May;83(10):3427-31. doi: 10.1073/pnas.83.10.3427.
9
Modification of T cell receptor beta chain gene: the J2 region but not the J1 region of the T cell receptor beta chain gene is hypomethylated in human B cells and monocytes.T细胞受体β链基因的修饰：在人类B细胞和单核细胞中，T细胞受体β链基因的J2区域而非J1区域发生低甲基化。
Nucleic Acids Res. 1988 Mar 25;16(5):2149-63. doi: 10.1093/nar/16.5.2149.
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