Tutt M M, Kuziel W A, Hackett J, Bennett M, Tucker P W, Kumar V
J Immunol. 1986 Nov 1;137(9):2998-3001.
Murine natural killer (NK) cells were purified by sorting with the NK cell-specific monoclonal antibody anti-NK-1.1. Sorted NK-1.1+ cells contained all splenic NK activity and could be propagated in human recombinant IL 2 for 3 to 4 wk. Short-term cultured NK-1.1+ cells maintained the cell-surface phenotype, morphologic appearance, and lytic activity characteristic of freshly isolated splenic NK cells. RNA extracted from this purified and propagated population of NK cells was probed for T cell receptor gene transcripts by Northern blot analysis. No functional transcripts of the alpha-, beta-, or gamma-chain genes of the T cell receptor were detected. These data dissociate NK cells from mature cytotoxic T cells, as well as the earliest identifiable cells committed to the T lineage.
通过用NK细胞特异性单克隆抗体抗NK-1.1进行分选，纯化小鼠自然杀伤（NK）细胞。分选得到的NK-1.1+细胞包含所有脾脏NK活性，并且可以在人重组白细胞介素2中培养3至4周。短期培养的NK-1.1+细胞维持了新鲜分离的脾脏NK细胞的细胞表面表型、形态外观和裂解活性特征。通过Northern印迹分析，用从这种纯化并扩增的NK细胞群体中提取的RNA探测T细胞受体基因转录本。未检测到T细胞受体α、β或γ链基因的功能性转录本。这些数据将NK细胞与成熟的细胞毒性T细胞以及最早可识别的T谱系细胞区分开来。
