Berkeleylactones and a Citreohybriddione Analogue from .

In 2017 we reported the isolation and characterization of berkeleylactones A-H and A26771B from a coculture of two extremophilic sp. isolated from an acid mine waste lake. Berkeleylactone A exhibited potent activity against several strains of multi-drug-resistant and . A26771B, which is related to the berkeleylactones, also exhibited antibiotic activity. Although the berkeleylactones were novel compounds, A26771B was originally isolated by scientists at Eli Lilly Company from and reported in1977. We recently obtained and grew it in axenic culture. We isolated five new berkeleylactones ( and -), two berkeley-γ-lactones ( and ), and citreohybriddional (), as well as the known compounds A26771B (), berkeleylactone E (), and gliovictin. The structures of the novel compounds were deduced from analysis of spectral data. Compounds and - are 16-membered macrolides, while and are γ-lactones that share the hexadecanoic acid skeleton. A26771B () and berkeleylactone I () were active against several strains of , including four multi-drug-resistant strains. Berkeleylactone N () was active only against .