Fellow Master degree. Postgraduate Program in Interdisciplinary Surgical Science - Surgery Department - Universidade Federal de São Paulo (UNIFESP) - São Paulo (SP), Brazil.
Fellow PhD degree. Immunology Department - Universidade Federal Fluminense (UFF) - Rio de Janeiro (RJ), Brazil.
Acta Cir Bras. 2021 Nov 29;36(10):e361008. doi: 10.1590/ACB361008. eCollection 2021.
To evaluate the morphological effects of a low-protein diet during maternal lactation on the offspring's thoracic aorta.
Two female Wistar rats were mated with male of the same species at 4 months of age. Until the birth of the pups, all animals received commercial rat chow. After giving birth, the puerperal females were divided into two groups and adjusted the litter to five puppies per group: a control group that received commercial feed, and an experimental group whose diet included the same amount of calories, but 8% lower protein content. All animals' masses were measured throughout the lactation period, and the pups were euthanized after weaning at 21 days of age. The thoracic aorta was removed, histologically processed and stained with Weigert's resorcin-fuchsin for histomorphometric analysis of tunica media thickness.
Although both groups were born with similar body mass, during the 21 days of lactation the restricted protein group gained only 39% of the body mass of the control group. Histomorphometric analysis revealed that the restricted protein group had a significantly lower mean tunica media thickness than the control group.
A low-protein diet for nursing mothers influences mass gain and aortic tunica media thickness in their offspring.
评估哺乳期低蛋白饮食对后代胸主动脉形态的影响。
4 月龄时，将两只雌性 Wistar 大鼠与同种类雄性大鼠交配。在幼崽出生前，所有动物均接受商业大鼠饲料喂养。分娩后，将哺乳期雌性大鼠分为两组，并将每窝幼崽调整为五只：对照组接受商业饲料，实验组饲料的热量相同，但蛋白质含量低 8%。在整个哺乳期内测量所有动物的体重，幼崽在 21 日龄断奶时处死。取出胸主动脉，进行组织学处理，并使用 Weigert 间苯二酚品红染色进行中膜厚度的组织形态计量学分析。
尽管两组动物出生时的体重相似，但在 21 天的哺乳期内，限制蛋白组仅获得了对照组体重的 39%。组织形态计量学分析显示，限制蛋白组的中膜厚度均值明显低于对照组。
哺乳期母亲的低蛋白饮食会影响其后代的体重增加和主动脉中膜厚度。
