紫花野芝麻的体外抗溶血作用、体内抗炎作用和体外抗氧化活性
In vitro Anti-hemolytic Effect, in vivo Anti-inflammatory and in vitro Anti-oxidant Activity of Anchusa azurea Mill.
机构信息
Laboratory of Applied Biochemistry, Department of Biology, Faculty of Sciences, University Ferhat Abbas of Setif 1, Setif 19000, Algeria.
Hospitalo-Universitary Centre (CHU) of Setif, Setif 19000, Algeria.
出版信息
Antiinflamm Antiallergy Agents Med Chem. 2022;21(1):24-33. doi: 10.2174/1871523020666211201162917.
BACKGROUND
Anchusa azurea Mill. (AA) is a medicinal plant largely used traditionally in folk medicine in Algeria; it is locally named hamham. It is effective in the treatment of various diseases.
OBJECTIVES
The aim of the present study is to determine the antioxidant, anti-inflammatory, and anti- hemolytic effects of phenolic fractions from Anchusa azurea Mill.
METHODS
In this study, various extracts from Anchusa azurea Mill. (AA) using solvents with increasing polarity were prepared. The quantification of polyphenols and flavonoids was determined. The anti-radical activity of the different extracts was evaluated using DPPH and by measuring the inhibition of the oxidative degradation of β-carotene. The In-vitro antihemolytic effect of the plant extracts is determined (CrE, ChE, AcE, and AqE). For each extract, four concentrations were tested: 10.59, 21.18, 42.37, 84.74 μg/ml. Vitamin C is used as a standard. The free-radical attack was measured by measuring the HT (Half-Hemolysis Time). The anti-inflammatory effect using PMA on mice of the methanolic extract (CrE) was evaluated.
RESULTS
The quantification of polyphenols and flavonoids showed that ethyl acetate extract (AcE) contains a higher amount of polyphenols. However, chloroform extract (ChE) presents a higher amount of flavonoids. AcE showed an important scavenging activity using the DPPH radical (IC= 68.35 μg/ml). The results showed that AcE also exhibited a significant inhibition effect on the oxidation of β-carotene/linoleic acid (84.33 %). All extracts increased the HT50 values (Half-Hemolysis Time) in a dose-dependent manner. The three highest concentrations (21.18, 42.37, and 84.74 μg / ml) of ChE caused a very significant delay (p ≤ 0.001) of hemolysis compared to the negative control and the positive control "VIT C". The anti-inflammatory effect of using PMA on mice showed that the methanolic extract (CrE) of AA reduced the weight of the ear edema.
CONCLUSION
This plant has a strong pharmacological power, which supports its traditional medicinal use.
背景
天蓝绣球（AA）是一种药用植物，在阿尔及利亚传统医学中被广泛应用，当地称之为 hamham。它在治疗各种疾病方面非常有效。
目的
本研究旨在确定天蓝绣球（AA）酚类部位的抗氧化、抗炎和抗溶血作用。
方法
本研究采用不同极性溶剂从天蓝绣球（AA）中提取，对提取物中的多酚和类黄酮进行定量分析。采用 DPPH 自由基清除法和β-胡萝卜素氧化降解抑制法评价不同提取物的自由基清除活性。通过测定植物提取物对 CrE、ChE、AcE 和 AqE 的抗溶血作用来确定其体外抗溶血效果。对每种提取物进行四个浓度（10.59、21.18、42.37 和 84.74μg/ml）的测试，维生素 C 作为标准。通过测定 HT（半溶血时间）来测量自由基攻击。采用 PMA 评价 AA 甲醇提取物（CrE）对小鼠的抗炎作用。
结果
多酚和类黄酮的定量分析表明，乙酸乙酯提取物（AcE）含有较高量的多酚，而氯仿提取物（ChE）含有较高量的类黄酮。AcE 对 DPPH 自由基具有很强的清除活性（IC=68.35μg/ml）。结果表明，AcE 对β-胡萝卜素/亚油酸的氧化也有显著的抑制作用（84.33%）。所有提取物均能使 HT50 值（半溶血时间）呈剂量依赖性增加。ChE 的三个最高浓度（21.18、42.37 和 84.74μg/ml）与阴性对照和阳性对照“VIT C”相比，溶血时间显著延长（p≤0.001）。用 PMA 处理小鼠显示，天蓝绣球的甲醇提取物（CrE）可减轻耳水肿的重量。
结论
该植物具有较强的药理作用，支持其传统药用价值。
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