BCYE 培养基在环境水样中军团菌检测的应用：对 ISO 11731:2017 标准的适当更新？
The use of BCYE medium for the detection of Legionella in environmental water samples: an appropriate update to ISO 11731:2017 standard?
机构信息
Department of Public Health and Pediatrics, University of Turin, Turin, Italy.
Department of Public Health and Pediatrics, University of Turin, Turin, Italy.
出版信息
Diagn Microbiol Infect Dis. 2022 Feb;102(2):115593. doi: 10.1016/j.diagmicrobio.2021.115593. Epub 2021 Nov 7.
We evaluated the diagnostic performances of 2 media (BCYE, MWY) on 951 Legionella-positive hospital water samples. MWY allowed detecting Legionella in 89.2% of samples, but in 10.8% (103/951) Legionella was found on BCYE plates only. In samples where Legionella was isolated with other microorganisms (663/951), MWY was essential to detect the majority of positive samples (349/663, 52.6%), as fewer plates resulted unreadable; however, in those containing Legionella only, a higher frequency of positive samples was recorded with BCYE (94.8%, 273/288) compared to MWY (85.1%, 245/288). Considering the 484 concordant positive samples, overall Legionella counts were significantly higher on BCYE (P = 0.0029), with 47% of samples showing higher counts on BCYE compared to MWY plates. Furthermore, discordant samples (positive on only one medium) showed different relative proportions between Legionella pneumophila and non-pneumophila, the latter being found more frequently on BCYE only (P = 0.0296).Our findings confirm the appropriateness of the ISO 11731:2017 update.
我们评估了 2 种培养基（BCYE、MWY）在 951 份 Legionella 阳性医院水样中的诊断性能。MWY 允许在 89.2%的样本中检测到 Legionella，但在 10.8%（103/951）的样本中仅在 BCYE 平板上发现 Legionella。在与其他微生物分离 Legionella 的样本中（663/951），MWY 对于检测大多数阳性样本至关重要（349/663，52.6%），因为较少的平板变得不可读；然而，在仅含有 Legionella 的样本中，BCYE 记录的阳性样本频率更高（94.8%，273/288），而 MWY 为 85.1%（245/288）。考虑到 484 个一致的阳性样本，BCYE 上的总 Legionella 计数明显更高（P=0.0029），与 MWY 平板相比，有 47%的样本显示 BCYE 上的计数更高。此外，不一致的样本（仅在一种培养基上呈阳性）显示出嗜肺军团菌和非嗜肺军团菌之间不同的相对比例，后者仅在 BCYE 上更频繁地发现（P=0.0296）。我们的发现证实了 ISO 11731:2017 更新的适当性。
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