Servicio de Microbiología, Hospital General Universitario de Valencia, Valencia, Spain; Facultad de Medicina, Universidad Católica de Valencia, Valencia, Spain.
Servicio de Microbiología, Hospital General Universitario de Valencia, Valencia, Spain.
Enferm Infecc Microbiol Clin (Engl Ed). 2024 Aug-Sep;42(7):373-376. doi: 10.1016/j.eimce.2024.05.007. Epub 2024 May 15.
Water sample culturing is the reference method to detect Legionella spp. in sanitary facilities. Until 2017, UNE-EN ISO 11731 only included the GVPC medium, which inhibits interfering microbiota but hinders the growth of Legionella spp. To improve its recovery, the new standard incorporates the BCYE medium into the working protocol.
We inoculated 1306 sanitary water samples onto BCYE and GVPC according to an accredited internal procedure. We compared the number of cfu/L of Legionella spp. detected in both media.
The median in BCYE was 2000 cfu/L higher than in GVPC (P = .000). In the presence of high amounts of interfering microbiota, both media were similar; in the absence or low interfering microbiota BCYE was four times more sensitive than GVPC (P = .000).
Including BCYE in the analysis of sanitary water significantly improves the recovery of Legionella spp. in low contaminated samples.
水样培养是检测卫生设施中军团菌属的参考方法。直到 2017 年，UNE-EN ISO 11731 仅包括 GVPC 培养基，该培养基可抑制干扰微生物群，但阻碍军团菌属的生长。为了提高其回收率，新标准将 BCYE 培养基纳入工作方案。
我们根据认可的内部程序将 1306 个卫生水样接种到 BCYE 和 GVPC 上。我们比较了两种培养基中检测到的军团菌属的 cfu/L 数量。
BCYE 中的中位数比 GVPC 高 2000 cfu/L（P=0.000）。在存在大量干扰微生物群的情况下，两种培养基相似；在干扰微生物群不存在或低的情况下，BCYE 比 GVPC 敏感四倍（P=0.000）。
在卫生水分析中纳入 BCYE 可显著提高低污染样本中军团菌属的回收率。
