School of Pharmacy, Nihon University.
Medical Mycology Research Center, Chiba University.
Med Mycol J. 2021;62(4):71-78. doi: 10.3314/mmj.21-00014.
Aspergillus fumigatus-related species are responsible for causing aspergillosis, which is a fatal infectious disease. Recently, there has been a series of reports of A. fumigatus-related species that are resistant to azole drugs used in clinical practice for the treatment of fungal infections. Some of these species have been isolated from outdoor environments. Testing the drug susceptibility of the strains from outdoor environments, therefore, is important. In this study, we isolated and cultured 72 strains of A. fumigatus-related species from the outdoor environment in Japan. The isolates identified via morphological observation and molecular phylogenetic analysis were Aspergillus felis, Aspergillus lentulus, Aspergillus pseudoviridinutans, Aspergillus udagawae, and Aspergillus wyomingensis. The results of the drug susceptibility testing revealed that A. felis (6 of 14 strains) and A. pseudoviridinutans (13 of 17 strains) were resistant to itraconazole (ITCZ), with 4 mg/L or higher minimum inhibitory concentrations (MICs). The voriconazole (VRCZ)-resistant strains with 4 mg/L or higher MICs were A. felis (14 of 14), A. lentulus (4 of 4), A. pseudoviridinutans (15 of 17), A. udagawae (23 of 34), A. wyomingensis (1 of 3), and A. pseudoviridinutans (1 of 3). Among them, A. felis (1 of 14) and A. pseudoviridinutans (7 of 17) demonstrated 8 mg/L or higher MICs for ITCZ and VRCZ. These results indicate that A. fumigatus-related species resistant to ITCZ and VRCZ are widely distributed in outdoor environments in Japan.
烟曲霉相关种是引起曲霉菌病的原因，这是一种致命的传染病。最近，有一系列关于烟曲霉相关种对唑类药物耐药的报道，这些药物用于治疗真菌感染。其中一些种已从室外环境中分离出来。因此，测试来自室外环境的菌株的药敏性很重要。在这项研究中，我们从日本的室外环境中分离和培养了 72 株烟曲霉相关种。通过形态观察和分子系统发育分析鉴定的分离物为费氏曲霉、 Lentulus 曲霉、拟青霉、乌达瓦曲霉和怀俄明曲霉。药敏试验结果显示，费氏曲霉（14 株中的 6 株）和拟青霉（17 株中的 13 株）对伊曲康唑（ITCZ）耐药，最低抑菌浓度（MIC）为 4 mg/L 或更高。MIC 为 4 mg/L 或更高的伏立康唑（VRCZ）耐药株有费氏曲霉（14 株中的 14 株）、 Lentulus 曲霉（4 株中的 4 株）、拟青霉（17 株中的 15 株）、乌达瓦曲霉（34 株中的 23 株）、怀俄明曲霉（3 株中的 1 株）和拟青霉（3 株中的 1 株）。其中，费氏曲霉（14 株中的 1 株）和拟青霉（17 株中的 7 株）对 ITCZ 和 VRCZ 的 MIC 为 8 mg/L 或更高。这些结果表明，对 ITCZ 和 VRCZ 耐药的烟曲霉相关种在日本的室外环境中广泛分布。
