Luo Hongcheng, Yi Tingzhuang, Huang Deyou, Chen Xiaoping, Li Xu, Wan Qianquan, Huang Haineng, Huang Huadong, Wei Hongyu, Song Ye, Que Tianshi, Hu Rentong, Huang Huatuo, Luo Kunxiang, Li Chuanyu, Qin Chengjian, Zheng Chuanhua, Lan Chuanliu, Chen Wencheng, Zhou Dan, Luo Qisheng
Department of Laboratory Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000 Guangxi, China.
The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Mol Ther Nucleic Acids. 2021 Sep 7;26:1255-1269. doi: 10.1016/j.omtn.2021.08.034. eCollection 2021 Dec 3.
Glioblastoma has been identified as the most common and aggressive primary brain tumor in adults. Recently, it has been found that cisplatin (DDP) treatment is a common chemotherapeutic method for GBM patients. circ_PTN (ID number: hsa_circ_0003949) is a newly found circular (circRNA) which has been proved to be highly expressed in GBM cells, while its role in GBM remains unclear. Therefore, our study focused on investigating the role of circ_PTN in the DDP resistance of GBM cells. The expression of circ_PTN in DDP-sensitive and DDP-resistant GBM cells was detected in our assay. Functional experiments were utilized to unveil the effects of circ_PTN on the DDP resistance of GBM cells. Moreover, mechanism assays were conducted to confirm the mechanism of how circ_PTN affected the DDP resistance of GBM cells. According to the results, we found that circ_PTN promoted the DDP resistance of GBM cells through activation of the PI3K/AKT pathway. Moreover, circ_PTN silencing inhibited the DDP resistance of GBM tumors . To conclude, our study unveiled the influence of circ_PTN on the DDP resistance of GBM cells, which might provide a therapeutic target for GBM treatment via DDP.
胶质母细胞瘤已被确定为成人中最常见且侵袭性最强的原发性脑肿瘤。最近，人们发现顺铂（DDP）治疗是胶质母细胞瘤患者常用的化疗方法。circ_PTN（编号：hsa_circ_0003949）是一种新发现的环状RNA（circRNA），已被证明在胶质母细胞瘤细胞中高表达，但其在胶质母细胞瘤中的作用仍不清楚。因此，我们的研究重点是探讨circ_PTN在胶质母细胞瘤细胞顺铂耐药中的作用。在我们的实验中检测了circ_PTN在顺铂敏感和耐药的胶质母细胞瘤细胞中的表达。利用功能实验揭示circ_PTN对胶质母细胞瘤细胞顺铂耐药性的影响。此外，还进行了机制分析，以确认circ_PTN影响胶质母细胞瘤细胞顺铂耐药性的机制。根据结果，我们发现circ_PTN通过激活PI3K/AKT通路促进了胶质母细胞瘤细胞的顺铂耐药性。此外，circ_PTN沉默抑制了胶质母细胞瘤肿瘤的顺铂耐药性。总之，我们的研究揭示了circ_PTN对胶质母细胞瘤细胞顺铂耐药性的影响，这可能为通过顺铂治疗胶质母细胞瘤提供一个治疗靶点。
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