朗缪尔-谢弗沉积法构建不对称脂多糖稀疏固定脂质双层。

Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely Tethered Lipid Bilayer.

机构信息

School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia.

Australian Centre for Neutron Scattering, Australian Nuclear Science and Technology Organisation, Lucas Heights, NSW, Australia.

出版信息

Methods Mol Biol. 2022;2402:21-30. doi: 10.1007/978-1-0716-1843-1_3.

DOI:10.1007/978-1-0716-1843-1_3

PMID:34854033

Abstract

Because they are firmly anchored to a noble metal substrate, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cranfield et al. Biophys J 106:182-189, 2014). The challenge to rapidly create asymmetrical tBLMs that include a lipopolysaccharide outer leaflet for bacterial model membrane research can be overcome by the use of a Langmuir-Schaefer deposition protocol. Here, we describe the procedures required to assemble and test asymmetric lipopolysaccharide (LPS) tethered lipid bilayers.

摘要

由于它们牢固地锚定在贵金属基底上，束缚双层脂质膜（tBLM）比支撑脂质膜（如黑质脂质膜（BLM））更加坚固（Cranfield 等人，《生物物理学杂志》106:182-189，2014）。通过使用Langmuir-Schaefer 沉积方案，可以克服快速创建包括脂多糖外叶的不对称 tBLM 以用于细菌模型膜研究的挑战。在这里，我们描述组装和测试不对称脂多糖（LPS）束缚脂质双层所需的程序。