使用连接双层脂膜（tBLM）测量离子传输的活化能。

Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs).

机构信息

School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW, Australia.

SDx Tethered Membranes Pty Ltd, Sydney, NSW, Australia.

出版信息

Methods Mol Biol. 2022;2402:71-79. doi: 10.1007/978-1-0716-1843-1_6.

DOI:10.1007/978-1-0716-1843-1_6

PMID:34854036

Abstract

Model lipid bilayers tethered to a gold substrate with molecular tethers are constructed. The conductance versus temperature dependence curve is then obtained. Here, a method to measure the activation energy for translocation of an ion through existing transmembrane pores in a sparsely tethered bilayer lipid membranes is presented.

摘要

构建了通过分子系链固定在金基底上的模型类脂双层。然后得到电导随温度的依赖关系曲线。本文提出了一种测量离子通过稀疏固定在双层脂膜上的跨膜孔道迁移的活化能的方法。