Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada.
Department of Chemistry, University of Tennessee, Knoxville, TN, USA.
Methods Mol Biol. 2022;2402:163-177. doi: 10.1007/978-1-0716-1843-1_14.
Lipid domains in model membranes are routinely studied to provide insight into the physical interactions that drive raft formation in cellular membranes. Using small angle neutron scattering, contrast-matching techniques enable the detection of lipid domains ranging from tens to hundreds of nanometers which are not accessible to other techniques without the use of extrinsic probes. Here, we describe a probe-free experimental approach and model-free analysis to identify lipid domains in freely floating vesicles of ternary phase separating lipid mixtures.
在模型膜中研究脂筏，以深入了解驱动细胞膜中筏形成的物理相互作用。使用小角中子散射，对比匹配技术可以检测到范围从几十到几百纳米的脂筏，而不使用外源性探针的其他技术则无法检测到。在这里，我们描述了一种无探针的实验方法和无模型分析，以识别三元相分离脂质混合物的自由浮动囊泡中的脂筏。
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