Marquardt Drew, Heberle Frederick A, Nickels Jonathan D, Pabst Georg, Katsaras John
University of Graz, Institute of Molecular Biosciences, Biophysics Division, NAWI Graz, Humboldtstr. 50/III, Graz, Austria.
Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
Soft Matter. 2015 Dec 21;11(47):9055-72. doi: 10.1039/c5sm01807b.
In order to understand the biological role of lipids in cell membranes, it is necessary to determine the mesoscopic structure of well-defined model membrane systems. Neutron and X-ray scattering are non-invasive, probe-free techniques that have been used extensively in such systems to probe length scales ranging from angstroms to microns, and dynamics occurring over picosecond to millisecond time scales. Recent developments in the area of phase separated lipid systems mimicking membrane rafts will be presented, and the underlying concepts of the different scattering techniques used to study them will be discussed in detail.
为了理解脂质在细胞膜中的生物学作用，有必要确定明确的模型膜系统的介观结构。中子散射和X射线散射是无创、无探针技术，已广泛应用于此类系统，以探测从埃到微米的长度尺度，以及在皮秒到毫秒时间尺度上发生的动力学过程。将介绍模拟膜筏的相分离脂质系统领域的最新进展，并详细讨论用于研究它们的不同散射技术的基本概念。
