Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Warsaw, Poland.
Methods Mol Biol. 2022;2402:199-207. doi: 10.1007/978-1-0716-1843-1_16.
Floating bilayer lipid membranes (fBLMs) immobilized on metallic surfaces provide a convenient model mimicking the cell membranes due to the effective hydration of lipid polar heads in a proximal leaflet and the possibility to generate the potential gradient across the membrane. This chapter describes the protocol for the measurement of interfacial water separating the floating bilayer lipid membrane from the solid support using surface-enhanced infrared absorption spectroscopy (SEIRAS) under electrochemical control.
固定在金属表面上的双层脂质膜（fBLM）由于近脂双层中脂质极性头部的有效水合作用以及在膜两侧产生电势梯度的可能性，为模拟细胞膜提供了一种便利的模型。本章描述了在电化学控制下使用表面增强红外吸收光谱（SEIRAS）测量将双层脂质膜与固体基底隔开的界面水的实验方案。
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