Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT-La Plata, CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.
Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
Methods Mol Biol. 2022;2402:243-256. doi: 10.1007/978-1-0716-1843-1_19.
The development of new strategies for achieving stable asymmetric membrane models has turned interleaflet lipid asymmetry into a topic of major interest. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophisticated equipment. Here we describe a protocol for preparing asymmetric supported lipid bilayers mimicking membrane rafts by cyclodextrin-mediated lipid exchange and the main guidelines for obtaining structural information and quantitative measures of their mechanical properties using Atomic force microscopy and Force spectroscopy; two powerful techniques that allow membrane characterization at the nanoscale.
开发新策略以实现稳定的不对称膜模型，使双层脂类不对称性成为主要关注的课题。环糊精介导的脂质交换是一种简单且多功能的方法，可用于制备不对称膜模型，而无需复杂的设备。在此，我们描述了一种使用环糊精介导的脂质交换制备模拟膜筏的不对称支撑脂双层的方案，以及使用原子力显微镜和力谱学获取结构信息和其机械性能定量测量的主要指南；这两种强大的技术可在纳米尺度上进行膜特性分析。
