Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Center for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health Republic of Indonesia, Jakarta 10560, Indonesia.
J Vet Sci. 2021 Nov;22(6):e86. doi: 10.4142/jvs.2021.22.e86.
Conditioned medium is the medium obtained from certain cultured cells and contained secretome from the cells. The secretome, which can be in the form of growth factors, cytokines, exosomes, or other proteins secreted by the cells, can induce the differentiation of cells that still have pluripotent or multipotent properties.
This study examined the effects of conditioned medium derived from E17 rat brain cells on cells with pluripotent properties.
The conditioned medium used in this study originated from E17 rat brain cells. The CM was used to induce the differentiation of primary colonies of mice blastocysts. Primary colonies were stained with alkaline phosphatase to analyze the pluripotency. The morphological changes in the colonies were examined, and the colonies were stained with GFAP and Neu-N markers on days two and seven after adding the conditioned medium.
The conditioned medium could differentiate the primary colony, beginning with the formation of embryoid-body-like structure; round GFAP positive cells were identified. Finally, neuron-like cells testing positive for Neu-N were observed on the seventh day after adding the conditioned medium.
Conditioned medium from different species, in this case, E17 rat brain cells, induced and promoted the differentiation of the primary colony from mice blastocysts into neuron-like cells. The addition of CM mediated neurite growth in the differentiation process.
条件培养基是从特定培养细胞中获得的培养基，其中包含细胞分泌的外泌体。这些分泌产物可以是生长因子、细胞因子、外泌体或其他由细胞分泌的蛋白质，它们可以诱导仍具有多能性或多能性的细胞分化。
本研究考察了来自 E17 大鼠脑细胞的条件培养基对具有多能性的细胞的影响。
本研究中使用的条件培养基来源于 E17 大鼠脑细胞。使用 CM 诱导小鼠囊胚原代集落的分化。用碱性磷酸酶染色分析集落的多能性。观察集落的形态变化，并在添加条件培养基后的第 2 天和第 7 天用 GFAP 和 Neu-N 标志物对集落进行染色。
条件培养基可诱导原代集落分化，首先形成类胚体样结构；鉴定出圆形 GFAP 阳性细胞。最后，在添加条件培养基后的第 7 天观察到神经元样细胞 Neu-N 阳性。
不同物种的条件培养基，在这种情况下是 E17 大鼠脑细胞，诱导并促进了来自小鼠囊胚的原代集落向神经元样细胞的分化。CM 的添加介导了分化过程中的神经突生长。
