Olesen S P
Brain Res. 1986 Mar 12;368(1):24-9. doi: 10.1016/0006-8993(86)91038-3.
The influence of lack of oxygen and metabolic inhibitors on the integrity of cerebral microvascular endothelium is undefined due to conflicting experimental evidence. A newly developed technique for continuous measurement of the electrical resistance of the microvascular endothelium was applied to the pial venules of the frog subjected to severe hypoxia and inhibition of endothelial ATP-production by means of cyanide (1 mM) and iodo-acetate (1 mM) for periods of 15 min. Severe hypoxia as well as application of the metabolic inhibitors decreased the electrical resistance within 2-5 min. The effect of the glycolysis-blocking agent, iodo-acetate, was particularly powerful. The maximal response, obtained in a 15 min period of exposure, was a 30-50% resistance decrease, corresponding to a 50-100% increase in the permeability to small ions. The rapid rise in permeability demonstrates that the cerebral endothelial cells depend critically on uninterrupted energy conversion to maintain the barrier function.
由于实验证据相互矛盾，缺氧和代谢抑制剂对脑微血管内皮完整性的影响尚不清楚。一种新开发的连续测量微血管内皮电阻的技术被应用于青蛙的软脑膜小静脉，使其遭受严重缺氧，并通过氰化物（1 mM）和碘乙酸盐（1 mM）抑制内皮细胞ATP生成，持续15分钟。严重缺氧以及应用代谢抑制剂在2 - 5分钟内降低了电阻。糖酵解阻断剂碘乙酸盐的作用尤为显著。在15分钟的暴露期内获得的最大反应是电阻降低30 - 50%，这相当于对小离子的通透性增加50 - 100%。通透性的快速增加表明脑内皮细胞严重依赖不间断的能量转换来维持屏障功能。
