Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs of P. R. China, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, P. R. China.
Division of Toxicology, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
J Agric Food Chem. 2021 Dec 22;69(50):15384-15392. doi: 10.1021/acs.jafc.1c05278. Epub 2021 Dec 2.
To assess potential differences in the intestinal microbial metabolism of deoxynivalenol (DON) and its acetylated forms 3-Ac-DON and 15-Ac-DON, anaerobic incubations with intestinal contents from chickens were conducted. Quantitative microbiota characterization was obtained by 16S rRNA sequencing. The data showed substantial differences in the level of different toxin conversions by the microbiota from the different intestinal segments. The transformation rate of DON to its metabolite DOM-1 decreased in the order of cecum > ileum > jejunum, and caecum contents could completely transform DON to DOM-1 within 24 h. However, no transformation appeared in the duodenum. For 3-Ac-DON, the deacetylation rate decreased as follows: duodenum > caecum > ileum > jejunum, and 100% deacetylation was observed in the duodenum within 24 h. The deacetylation of 15-Ac-DON decreased in the order of cecum > duodenum > ileum with no transformation in the jejunum. Some genera may contribute to the transformation of the toxins. Based on the kinetic parameters and their scaling to the situation, it was concluded that in the chicken small intestine, the deconjugation of both 3-Ac-DON and 15-Ac-DON will not likely be completed upon full transit. Whether this also holds for humans remains to be established.
为了评估脱氧雪腐镰刀菌烯醇（DON）及其乙酰化形式 3-Ac-DON 和 15-Ac-DON 在肠道微生物代谢中的潜在差异，我们进行了鸡肠道内容物的厌氧孵育。通过 16S rRNA 测序获得了定量微生物特征。数据显示，不同肠道段的微生物对不同毒素转化的水平存在显著差异。DON 向其代谢物 DOM-1 的转化速率按盲肠>回肠>空肠的顺序降低，盲肠内容物在 24 小时内可以完全将 DON 转化为 DOM-1。然而，十二指肠中没有出现转化。对于 3-Ac-DON，脱乙酰化率按以下顺序降低：十二指肠>盲肠>回肠>空肠，在 24 小时内，十二指肠中 100%脱乙酰化。15-Ac-DON 的脱乙酰化按盲肠>十二指肠>回肠的顺序降低，空肠中没有转化。一些属可能有助于毒素的转化。基于动力学参数及其对情况的扩展，得出的结论是，在鸡的小肠中，3-Ac-DON 和 15-Ac-DON 的去结合不太可能在完全转运时完成。这是否也适用于人类还有待确定。
