Carbone A, Piantelli M, Musiani P, Larocca L M, Revoltella R P, Ranelletti F O
Clin Exp Immunol. 1986 Jan;63(1):203-9.
Expression of 20-alpha-hydroxysteroid dehydrogenase (20 alpha-SDH), a putative T cell marker in the murine system, has been examined in human haematopoietic cells. Higher levels of enzymatic activity were expressed by human peripheral blood mononuclear cells (PBMC) in comparison with thymocytes. When PBMC were fractionated into T and non T cell subsets, the greatest values of 20 alpha-SDH activity were associated with the non T cell population. Cells from bone marrow exhibited lower levels of 20 alpha-SDH than PBMC and thymocytes. Both myeloid and lymphoid leukaemic cells expressed significant 20 alpha-SDH activity. In addition our data in U-937 and CM-S human cell lines indicate that cells belonging to the myelomonocytic precursor compartment constitutively expressed 20 alpha-SDH activity. Furthermore, this activity was less expressed when these cells were induced to monocyte-macrophage differentiation. In conclusion, our data in the human system indicate that 20 alpha-SDH should not be viewed as a lymphoid lineage-restricted marker enzyme.
在小鼠系统中，一种假定的T细胞标志物——20-α-羟基类固醇脱氢酶（20α-SDH）的表达情况已在人类造血细胞中进行了检测。与胸腺细胞相比，人类外周血单个核细胞（PBMC）表达出更高水平的酶活性。当将PBMC分离为T细胞和非T细胞亚群时，20α-SDH活性的最高值与非T细胞群体相关。骨髓细胞表现出的20α-SDH水平低于PBMC和胸腺细胞。髓系和淋巴系白血病细胞均表达出显著的20α-SDH活性。此外，我们在U-937和CM-S人细胞系中的数据表明，属于髓单核细胞前体区室的细胞组成性地表达20α-SDH活性。此外，当这些细胞被诱导分化为单核细胞-巨噬细胞时，这种活性表达减少。总之，我们在人类系统中的数据表明，20α-SDH不应被视为一种淋巴系限制性标志物酶。
