氨基酸精氨酸和一氧化氮在非阿片类亮啡肽类似物体外细胞保护作用机制中的作用。
Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro.
机构信息
Far Eastern State Medical University, Ministry of Health of the Russian Federation, Khabarovsk, Russia.
Khabarovsk Branch of the Far Eastern Research Center for Physiology and Pathology of Respiration - Research Institute for the Protection of Motherhood and Childhood, Khabarovsk, Russia.
出版信息
Bull Exp Biol Med. 2021 Dec;172(2):270-275. doi: 10.1007/s10517-021-05374-2. Epub 2021 Dec 2.
Incubation of primary culture of pulmonary fibroblasts with non-opiate analogue of leuenkephalin (NALE; Phe-D-Ala-Gly-Phe-Leu-Arg, 0.1 μM) reduced generation of superoxide anion-radical (by 20.7%) and decreased the number of p53 cells (by 40.2%) induced by exposure to HO (60 μM). The cytoprotective effect of NALE was potentiated by NO synthase inhibitor L-NAME (1 mM): the number of p53 cells decreased by 65.3% and morphometric parameters of the cell nuclei and nucleoli were improved. Incubation of pulmonary fibroblasts culture with peptide G (Phe-D-Ala-Gly-Phe-Leu-Gly, 0.1 μM) also significantly reduced the damaging effect of HO: the number of p53 cells decreased by 73.5%, the area of cell nuclei returned to normal, and generation of superoxide anion-radical decreased by 18.4%. These results indicate that C-terminal amino acid Arg and activation of NO synthase are not involved in the direct cytoprotective effect of NALE.
用非鸦片类似物亮啡肽（NALE；Phe-D-Ala-Gly-Phe-Leu-Arg，0.1μM）孵育肺成纤维细胞原代培养物可减少由暴露于 HO（60μM）引起的超氧阴离子自由基（减少 20.7%）和 p53 细胞（减少 40.2%）的产生。NO 合酶抑制剂 L-NAME（1mM）增强了 NALE 的细胞保护作用：p53 细胞减少 65.3%，细胞核和核仁的形态参数得到改善。用肽 G（Phe-D-Ala-Gly-Phe-Leu-Gly，0.1μM）孵育肺成纤维细胞培养物也显著降低了 HO 的损伤作用：p53 细胞减少 73.5%，细胞核面积恢复正常，超氧阴离子自由基的产生减少 18.4%。这些结果表明，C 末端氨基酸 Arg 和 NO 合酶的激活不参与 NALE 的直接细胞保护作用。
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