Fukamachi H, Mizuno T, Kim Y S
Experientia. 1986 Mar 15;42(3):312-5. doi: 10.1007/BF01942518.
The morphogenesis and cytodifferentiation of human colon cancer cells (LS174T and HT29) were examined by combining cancer cells with fetal rat digestive-tract mesenchyme in organ culture. LS174T cells migrated into the mesenchyme to form glandular structures composed of single columnar cells with their nuclei oriented basally, while HT29 cells formed cell masses with little lumen formation. Immunohistochemical studies with antibodies against carcinoembryonic antigen and secretory components showed that the composition of cell surface glycoproteins was not necessarily reversed to the normal type, even when neoplastic cells exhibited normal glandular structures.
通过在器官培养中将人结肠癌细胞（LS174T和HT29）与胎鼠消化道间充质结合，研究了人结肠癌细胞的形态发生和细胞分化。LS174T细胞迁移到间充质中，形成由单层柱状细胞组成的腺结构，细胞核位于基部，而HT29细胞形成细胞团，几乎没有管腔形成。用抗癌胚抗原和分泌成分的抗体进行免疫组织化学研究表明，即使肿瘤细胞呈现正常腺结构，细胞表面糖蛋白的组成也不一定会恢复到正常类型。
