Quintero Marynes, Blandón Lina M, Vidal Oscar M, Guzman Juan D, Gómez-Marín Jorge E, Patiño Albert D, Molina Diego A, Puerto-Castro Gloria M, Gómez-León Javier
Marine Bioprospecting Line, Evaluation and Use of Marine and Coastal Resources Program-VAR, Marine and Coastal Research Institute-INVEMAR, Santa Marta, Colombia.
Division of Health Sciences, Medicine Department, Universidad del Norte, Barranquilla, Colombia.
J Appl Microbiol. 2022 Apr;132(4):2705-2720. doi: 10.1111/jam.15397. Epub 2021 Dec 22.
To evaluate the biological activity of extracts from cultures of marine bacteria against Toxoplasma gondii and Mycobacterium tuberculosis.
Ethyl acetate extracts obtained from seven marine bacteria were tested against T. gondii GFP-RH and M. tuberculosis H37Rv. The cytotoxicity on HFF-1 cells was measured by a microplate resazurin fluorescent approach, and the haemolytic activity was determined photometrically. The extracts from Bacillus sp. (INV FIR35 and INV FIR48) affected the tachyzoite viability. The extracts from Bacillus, Pseudoalteromonas, Streptomyces and Micromonospora exhibited effects on infection and proliferation processes of parasite. Bacillus sp. INV FIR48 extract showed an minimum inhibitory concentration value of 50 µg ml against M. tuberculosis H37Rv. All the extracts exhibited relatively low toxicity to HFF-1 cells and the primary culture of erythrocytes, except Bacillus sp. INV FIR35, which decreased cell viability under 20%. Liquid chromatography coupled to mass spectrometry analysis of the most active bacterial extract Bacillus sp. INV FIR48 showed the presence of peptide metabolites related to surfactin.
The extract from culture of deep-sea Bacillus sp. INV FIR48 showed anti-T. gondii and anti-tuberculosis (TB) biological activity with low cytotoxicity. In addition, peptide metabolites were detected in the extract.
Toxoplasmosis and TB are among the most prevalent diseases worldwide, and the current treatment drugs exhibit side effects. This study confirm that marine bacteria are on hand sources of anti-infective natural products.
评估海洋细菌培养物提取物对刚地弓形虫和结核分枝杆菌的生物活性。
测试了从七种海洋细菌中获得的乙酸乙酯提取物对刚地弓形虫GFP-RH株和结核分枝杆菌H37Rv株的作用。采用微孔板刃天青荧光法测定对人包皮成纤维细胞（HFF-1）的细胞毒性，用光度法测定溶血活性。芽孢杆菌属（INV FIR35和INV FIR48）的提取物影响速殖子活力。芽孢杆菌属、假交替单胞菌属、链霉菌属和小单孢菌属的提取物对寄生虫的感染和增殖过程有影响。芽孢杆菌属INV FIR48提取物对结核分枝杆菌H37Rv的最低抑菌浓度值为50μg/ml。除芽孢杆菌属INV FIR35外，所有提取物对HFF-1细胞和红细胞原代培养物的毒性相对较低，INV FIR35使细胞活力降低至20%以下。对活性最强的细菌提取物芽孢杆菌属INV FIR48进行液相色谱-质谱联用分析，结果显示存在与表面活性素相关的肽类代谢产物。
深海芽孢杆菌属INV FIR48培养物的提取物具有抗刚地弓形虫和抗结核生物活性，且细胞毒性低。此外，提取物中检测到了肽类代谢产物。
弓形虫病和结核病是全球最普遍的疾病，目前的治疗药物有副作用。本研究证实海洋细菌是抗感染天然产物的现成来源。
