Nayak S K, Samples J R, Deg J K, Binder P S
Invest Ophthalmol Vis Sci. 1986 Apr;27(4):607-11.
Primate (baboon) corneal endothelial cells have been grown continuously in vitro for over 40 passages. Endothelial cells migrated from the explants within three to five days. Growth became confluent by 14 to 21 days; the cells in culture were polygonal in shape and formed a compact monolayer. In passages one to four, cells divided with a doubling time of 72 hours; this increased to 96 hours after the 16th passage. Even though the general morphological appearance was unchanged in passages greater than 10, the cells became irregular, exhibiting an enlarged and elongated profile. The addition of epidermal growth factor to the medium resulted in increased cell growth. Cultured cells were successfully transplanted onto Descemet's membrane of de-endothelialized baboon corneas. This study demonstrates that primate corneal endothelial cells can be grown continuously in tissue culture and subsequently transplanted in vitro.
灵长类动物（狒狒）角膜内皮细胞已在体外连续培养超过40代。内皮细胞在三到五天内从外植体迁移出来。到14至21天时生长融合；培养的细胞呈多边形，形成紧密的单层。在第1到4代中，细胞以72小时的倍增时间分裂；第16代后增加到96小时。尽管在超过10代后总体形态外观没有变化，但细胞变得不规则，呈现出增大和拉长的形态。向培养基中添加表皮生长因子导致细胞生长增加。培养的细胞成功移植到去内皮化狒狒角膜的后弹力层上。这项研究表明，灵长类动物角膜内皮细胞可以在组织培养中连续生长，随后进行体外移植。
