Raymond G M, Jumblatt M M, Bartels S P, Neufeld A H
Invest Ophthalmol Vis Sci. 1986 Apr;27(4):474-9.
Rabbit corneal endothelial cells were grown in tissue culture. Epidermal growth factor (EGF) increased the mitotic rate during the growth phase by 70% over control without affecting the plating efficiency. Within 48 hr of exposure to EGF, the endothelial cells became spindle-shaped. This morphological change was quantitated by morphometry; cells treated with EGF had a major axis 1.5 X larger than that of non-EGF treated cells. The spindle-shaped morphological change did not occur in response to other growth factors, was not related to cell density, and was reversible within 24 hr after removal of EGF from the media or subculture in the absence of EGF. The addition of 5-fluorouracil blocked cell division but did not affect the EGF-induced morphological change. The appearance of the endothelial cells following EGF stimulation is similar to migrating cells closing a wound in vivo.
兔角膜内皮细胞在组织培养中生长。表皮生长因子（EGF）使生长阶段的有丝分裂率比对照组提高了70%，且不影响接种效率。在暴露于EGF的48小时内，内皮细胞变成了纺锤形。这种形态变化通过形态测量进行定量；用EGF处理的细胞的长轴比未用EGF处理的细胞大1.5倍。纺锤形的形态变化不是对其他生长因子的反应，与细胞密度无关，并且在从培养基中去除EGF或在无EGF的条件下传代培养后24小时内是可逆的。添加5-氟尿嘧啶可阻断细胞分裂，但不影响EGF诱导的形态变化。EGF刺激后内皮细胞的外观类似于体内闭合伤口的迁移细胞。
