Harmel R P, Stanley M
J Pediatr Surg. 1986 Mar;21(3):214-7. doi: 10.1016/s0022-3468(86)80836-3.
We investigated the effect of cyclosporine on survival after intestinal transplantation between histoincompatible Brown Norway (AgB3/3) and Lewis (AgB1/1) rats. Intestinal grafts were primarily vascularized (by microsurgical techniques) and interposed isoperistaltically in the recipient's jejunum. All animals were weighed and observed daily. Survival of recipients given cyclosporine 20 mg/kg/d (112 +/- 92 days, n = 10) was significantly longer (P less than .02) than that of recipients given no drug (12 +/- 4 days, n = 8). Six out of ten cyclosporine-treated animals remained alive and well at the conclusion of the experiment. One of these was killed and showed no gross or microscopic evidence of rejection. The described experimental model involves a simple operative technique and minimal postoperative care and should permit systematic investigation of the detection and prevention of rejection.
我们研究了环孢素对组织不相容的棕色挪威大鼠（AgB3/3）和刘易斯大鼠（AgB1/1）之间肠道移植术后存活情况的影响。肠道移植物首先通过显微外科技术进行血管化处理，并以等蠕动方式置于受体空肠中。每天对所有动物称重并进行观察。给予20mg/kg/d环孢素的受体存活时间（112±92天，n = 10）明显长于未给药的受体（12±4天，n = 8）（P＜0.02）。在实验结束时，接受环孢素治疗的10只动物中有6只仍存活且状况良好。其中一只被处死，未发现明显的排斥反应或微观证据。所描述的实验模型涉及一种简单的手术技术和最少的术后护理，应该能够对排斥反应的检测和预防进行系统研究。
