Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, Bd Maurice de Broglie, 76821 Mont Saint Aignan Cedex, France; Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, 55 rue Saint-Germain, 27000 Évreux, France.
Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, 55 rue Saint-Germain, 27000 Évreux, France.
Mater Sci Eng C Mater Biol Appl. 2021 Dec;131:112479. doi: 10.1016/j.msec.2021.112479. Epub 2021 Oct 16.
Implanted biomaterials can be regarded in a cornerstone in the domain of bone surgery. Their surfaces are expected to fulfil two particular requirements: preventing the settlement and the development of bacteria, and stimulating bone cells in view to foster osseointegration. Therefore, a modern approach consists in the design of dual functional coatings with both antibacterial and osteogenic features. To this end, we developed ultrathin Layer-by-Layer (LbL) coatings composed of biocompatible polyelectrolytes, namely chondroitin sulfate A (CSA) and poly-l-lysine (PLL). The coatings were crosslinked with genipin (GnP), a natural and biocompatible crosslinking agent, to increase their resistance against environmental changes, and to confer them adequate mechanical properties with regards to bone cell behaviors. Antibacterial activity was obtained with nisin Z, an antimicrobial peptide (AMP), which is active against gram-positive bacteria. The coatings had a significant bactericidal impact upon Staphylococcus aureus, with fully maintained bone cell adhesion, proliferation and osteogenic differentiation.
植入生物材料被认为是骨外科领域的基石。它们的表面需要满足两个特殊要求：防止细菌的定居和发展，以及刺激成骨细胞以促进骨整合。因此，一种现代方法是设计具有抗菌和促成骨双重功能的涂层。为此，我们开发了由生物相容性聚电解质组成的超薄层层（LbL）涂层，即硫酸软骨素 A（CSA）和聚-L-赖氨酸（PLL）。这些涂层用京尼平（GnP）交联，京尼平是一种天然的生物相容性交联剂，以增加它们对环境变化的抵抗力，并赋予它们适当的机械性能，以适应骨细胞的行为。通过使用抗菌肽（AMP）尼生素 Z 获得了抗菌活性，尼生素 Z 对革兰氏阳性菌具有活性。这些涂层对金黄色葡萄球菌具有显著的杀菌作用，同时完全保持了细胞的黏附、增殖和成骨分化。
