Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, Bd Maurice de Broglie, 76821 Mont Saint Aignan Cedex, France; Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, 55 rue Saint-Germain, 27000 Évreux, France.
Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, 55 rue Saint-Germain, 27000 Évreux, France.
Colloids Surf B Biointerfaces. 2021 Dec;208:112121. doi: 10.1016/j.colsurfb.2021.112121. Epub 2021 Sep 20.
Some removable medical devices such as catheters and cardiovascular biomaterials require antiadhesive properties towards both prokaryotic and eukaryotic cells in order to prevent the tissues from infections upon implantation and, from alteration upon removal. In order to inhibit cell adhesion, we developed ultrathin hydrated Layer-by-Layer (LbL) coatings composed of biocompatible polyelectrolytes, namely chondroitin sulfate A (CSA) and poly-l-lysine (PLL). The coatings were crosslinked with genipin (GnP), a natural and biocompatible crosslinking agent, to increase their resistance against environmental changes. In order to confer antibacterial activity to the coatings, we proceeded to the electrostatically-driven immobilization of nisin Z, an antimicrobial peptide (AMP) active against gram-positive bacteria. The nisin-enriched coatings had a significantly increased anti-proliferative impact on fibroblasts, as well as a strong contact-killing activity against Staphylococcus aureus in the short and long term.
一些可移除的医疗设备，如导管和心血管生物材料，需要对原核细胞和真核细胞都具有抗粘附性，以防止植入后组织感染，并防止在移除时发生变化。为了抑制细胞黏附，我们开发了由生物相容性聚电解质组成的超薄水合层层（LbL）涂层，即硫酸软骨素 A（CSA）和聚-l-赖氨酸（PLL）。这些涂层用京尼平（GnP）交联，京尼平是一种天然的、生物相容的交联剂，以提高其对环境变化的抵抗力。为了赋予涂层抗菌活性，我们进行了静电固定化纳他霉素 Z，一种对革兰氏阳性菌有效的抗菌肽（AMP）。富含纳他霉素的涂层对成纤维细胞的增殖有明显的抑制作用，并且在短期和长期内对金黄色葡萄球菌具有强烈的接触杀灭活性。
