Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China; Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University, Hangzhou, 310018, China.
Talanta. 2022 Feb 1;238(Pt 1):122988. doi: 10.1016/j.talanta.2021.122988. Epub 2021 Oct 22.
The illegal adulteration of sulfur dioxide in natural healthcare products may lead to serious health problems, which raise an urgent demand of straightforward approach for detecting sulfur dioxide. In this paper, surface-enhanced Raman scattering (SERS) sensor with sample preparation apparatus for headspace adsorption of SO has been developed, which was successfully applied to detect illegal adulteration of sulfur dioxide in traditional Chinese medicine (TCM). Functional membrane substrate of Si@Ag@PEI composite was synthesized to enhance the adsorption and Raman signal of SO. A 3D-printed headspace extraction device was designed to adsorbed SO by Si@Ag@PEI membrane after micro-extraction of TCM samples in 15 min. The content of sulfur dioxide was subsequently quantitatively measured by SERS sensor. The linear range of sensor is between 2.5 and 250 mg/kg with limit of detection of 0.25 mg/kg, which is lower than the strictest standard of Chinese Pharmacopoeia (10 mg/kg). The proposed approach was used to detect the SO residue in TCMs including ginseng, Salvia miltiorrhiza, and bitter almonds. The fabricated sensor exhibited satisfied sensitivity and stability, which provide a simple approach for on-site detection of illegal adulteration of sulfur dioxide.
二氧化硫在天然保健品中的非法添加可能会导致严重的健康问题，这就迫切需要一种简单的方法来检测二氧化硫。本文开发了一种带有顶空吸附 SO 的样品制备装置的表面增强拉曼散射（SERS）传感器，成功应用于检测中药（TCM）中二氧化硫的非法添加。合成了 Si@Ag@PEI 复合功能膜基底，以增强 SO 的吸附和拉曼信号。设计了一种 3D 打印的顶空提取装置，在对 TCM 样品进行 15 分钟微提取后，用 Si@Ag@PEI 膜吸附 SO。随后通过 SERS 传感器定量测量二氧化硫的含量。传感器的线性范围在 2.5 到 250mg/kg 之间，检测限为 0.25mg/kg，低于中国药典（10mg/kg）的严格标准。该方法用于检测包括人参、丹参和苦杏仁在内的 TCM 中的二氧化硫残留。所制备的传感器表现出良好的灵敏度和稳定性，为现场检测二氧化硫的非法添加提供了一种简单的方法。
